磷酸二酯酶通过cAMP-PKA途径调控核盘菌菌核原基的形成  

Phosphodiesterase Regulates the Formation of Sclerotia Primordium of Sclerotinia sclerotiorum through cAMP-PKA Pathway

作　　者：吕蕊花 陈萌 赵倩 冯昭 史琳娜 张喜荣 LÜRuihua;CHEN Meng;ZHAO Qian;FENG Zhao;SHI Linna;ZHANG Xirong(College of Medical Technology,Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学医学技术学院,陕西咸阳712046

出　　处：《河南农业科学》2022年第9期99-104,共6页Journal of Henan Agricultural Sciences

基　　金：陕西省自然科学基础研究计划项目(2021JQ-725);陕西中医药大学学科创新团队项目(2019-YS04);陕西省教育厅一般专项科研计划项目(21JK0595)。

摘　　要：在PDA培养基中添加不同浓度的磷酸二酯酶(PDEs)抑制剂阿托品,检测其对核盘菌生长发育的影响,并采用qRT-PCR方法检测PDEs主效基因及cAMP-PKA通路中相关基因的表达情况,研究核盘菌PDEs在cAMP-PKA通路中的作用,为开发以PDEs为靶标的新型杀菌剂提供理论基础。结果表明,当阿托品添加浓度为4 mmol/L时,核盘菌菌丝生长速率和菌核形成均受到影响。从核盘菌基因库中检索出了8个PDEs,qRT-PCR结果显示,PDE1/2在核盘菌菌核形成过程中表达量较高。添加PDEs抑制剂后,cAMP-PKA通路中PDEs上游基因AC和RAS表达量上调,但PDE1、PDE2和下游因子PKA表达量均下降。由此可知,PDEs在核盘菌菌核形成方面具重要作用,PDEs抑制剂通过抑制PDE1/2的表达使得下游因子PKA表达量下降,进一步影响菌核原基的形成。To study the role of phosphodiesterase(PDEs)in the formation of sclerotia primordium of Sclerotinia sclerotiorum,and provide a theoretical basis for the development of new fungicides targeting PDEs,different concentrations of atropine,a PDEs inhibitor,were added to the PDA medium to detect its effects on the growth and development of Sclerotinia sclerotiorum,and qRT-PCR was used to detect the expression of major genes of PDEs and in the cAMP-PKA pathway.The results showed that the mycelial growth rate of Sclerotinia sclerotiorum and the formation of sclerotia were affected when the concentration of atropine was 4 mmol/L.Eight PDEs were retrieved from the Sclerotinia sclerotiorum gene bank,and qRT-PCR results showed that PDE1/2 were highly expressed during the formation of sclerotia.After the addition of PDEs inhibitors,the expressions of the upstream genes of PDEs(AC and RAS)in the cAMP-PKA pathway were up-regulated,but the expressions of PDE1,PDE2 and the downstream factor PKA were all decreased.It can be seen that PDEs play an important role in the formation of sclerotia of Sclerotinia sclerotiorum.PDEs inhibitors reduce the expression of downstream factor PKA by inhibiting the expression of PDE1/2,which further affects the formation of sclerotia primordium.

关 键 词：核盘菌 磷酸二酯酶 cAMP-PKA途径 菌核形成 

分 类 号：S432.44[农业科学—植物病理学]