不同肝损伤程度慢性乙型肝炎肝硬化患者血清Nrf2、HO-1水平的表达分析  

An analysis on the expression of serum Nrf2 and HO-1 in patients with chronic hepatitis B-related cirrhosis and different degrees of liver injury

作　　者：陈继德 何瑛[2] 王鹏森[2] 李健 冯华国 CHEN Ji-de;HE Ying;WANG Peng-sen;LI Jian;FENG Hua-guo(Department of Clinical Laboratory,Bishan of Chongqing Medical University Hospital,Chongqing 400030,China;Central Laboratory,Chongqing Public Health Medical Center,Chongqing 402760,China;Department of Clinical Laboratory,Jiangjin Central Hospital of Chongqing,Chongqing 402260,China)

机构地区：[1]重庆医科大学附属璧山医院检验科,402760 [2]重庆市公共卫生医疗救治中心中心实验室 [3]重庆市江津区中心医院检验科

出　　处：《肝脏》2022年第10期1102-1105,1119,共5页Chinese Hepatology

基　　金：重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0620)。

摘　　要：目的分析慢性乙型肝炎肝硬化患者不同肝损伤程度血清核因子E2相关因子2(Nrf2)、血红素氧合酶(HO-1)表达情况。方法选取2019年1月—2020年6月重庆医科大学附属璧山医院收治的95例慢性乙型肝炎肝硬化患者作为研究对象,于入院时评估所有患者的肝损伤程度(Child-Pugh分级)并分为轻度组、中度组、重度组,设计基线资料调查表,统计并比较3组患者的基线资料,重点分析乙型肝炎肝硬化患者入院时血清Nrf2、HO-1与肝损伤程度的关系。结果95例慢性乙型肝炎肝硬化患者Child-Pugh A级29例,B级35例,C级31例;重度组患者的血清Nrf2、HO-1水平依次低于中度组、轻度组,中度组患者的血清Nrf2、HO-1水平低于轻度组,差异有统计学意义(P<0.05),不同肝损伤程度慢性乙型肝炎肝硬化患者的基线资料比较差异无统计学意义(P>0.05);经Kendall's tau-b相关性分析发现,慢性乙型肝炎肝硬化患者肝损伤程度与血清Nrf2、HO-1水平呈负相关(r<0,P<0.05);多项logistic回归分析结果显示,血清Nrf2、HO-1异常表达可能与慢性乙型肝炎肝硬化肝损伤程度有关(OR<1,P<0.05)。结论入院时血清Nrf2、HO-1的异常低表达可能与慢性乙型肝炎肝硬化患者中、重度肝损伤有关。Objective To analyze the expression of serum nuclear factor E2 related factor 2(Nrf2) and heme oxygenase(HO-1) in patients with chronic hepatitis B-related cirrhosis with different degrees of liver injuries.Methods 95 patients with chronic hepatitis B-related cirrhosis treated in the hospital from January 2019 to June 2020 were selected as the research subjects.The degree of liver injury(Child-Pugh grade) of all patients was evaluated at admission and divided into a mild group,a moderate group and a severe group.The patients were then investigated with a designed baseline data questionnaire.The baseline data of patients in these three groups were counted and compared,along with analyses on the association between serum Nrf2,HO-1 and the degrees of liver injuries in the patients.Results Among 95 patients with chronic hepatitis B-related cirrhosis after assessment,29 were Child-Pugh grade A,35 were Child-Pugh grade B,and31 were Child-Pugh grade C.The levels of serum Nrf2 and HO-1 of patients in severe group were lower than those in moderate group and mild group,and the levels of serum Nrf2 and HO-1 of patients in moderate group were lower than those in mild group,the differences were statistically significant(P<0.05) .There was no statistic significant difference in the baseline data of patients with chronic hepatitis B-related cirrhosis with different degrees of liver injuries(P>0.05) .By Kendall’s tau-b correlation analysis,it was found that the degrees of liver injuries in patients with chronic hepatitis B cirrhosis was negatively correlated with the levels of serum Nrf2 and HO-1(r<0,P<0.05) .The results of multiple logistic regression analysis showed that the abnormal expression of serum Nrf2 and HO-1 may be related to the degrees of liver injuries in chronic hepatitis B cirrhosis(OR<1,P<0.05) .Conclusion The abnormal low expression of serum Nrf2 and HO-1 at admission may be related to moderate and severe liver injuries in patients with chronic hepatitis B-related cirrhosis.

关 键 词：慢性乙型肝炎 肝硬化 肝损伤 核因子E2相关因子2 血红素氧合酶 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]