五味子藤茎多糖小鼠经口最大耐受量的测定  

Determination of Oral Maximum Tolerance Dose of Schisandra chinensis Vine Stem Polysaccharide in Mice

作　　者：李贺[1] 陈红旭 牛胜男 陈建光[1] LI He;CHEN Hongxu;NIU Shengnan;CHEN Jianguang(Pharmacy College of Beihua University,Jilin 132013,China)

机构地区：[1]北华大学药学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2022年第6期763-767,共5页Journal of Beihua University（Natural Science）

基　　金：吉林省科技发展计划项目(20200404022YY);吉林省发改委产业技术与开发类项目(2020C033-2).

摘　　要：目的测定五味子藤茎多糖灌服小鼠经口最大耐受量(Maximum Tolerance Dose,MTD),并评估其安全性.方法将20只ICR小鼠随机平均分为空白组(CON)和藤茎多糖组(SCP),观察记录7 d内小鼠的一般状态、中毒及死亡情况;应用避暗实验、抓力实验、自主活动实验观察小鼠学习记忆能力、运动耐力等;计算脏器指数,并应用HE染色观察脏器损伤程度.结果SCP组与CON组比较,在7 d内,灌服SCP未导致小鼠死亡;生理反应、精神状态和行为活动等未见异常;其活动次数、站立次数、避暗错误次数、避暗潜伏期以及前肢握力的差异均无统计学意义(P>0.05).解剖后未见小鼠各脏器病变,心脏指数、肝脏指数、肺脏指数、脾脏指数、肾脏指数、脑器官指数差异均无统计学意义(P>0.05);病理切片显示各脏器结构均正常,无明显病变.结论SCP小鼠经口MTD为24 g/kg,在该剂量下,SCP对小鼠的生长、生理状态及行为无显著影响.Objective To determine the oral maximum tolerance dose(MTD)of Schisandra chinensis vine stem polysaccharide in mice and to evaluate its safety.Method Twenty ICR mice were evenly and randomly divided into blank group and vine polysaccharide group,and the general status,poisoning and death of mice were observed and recorded for 7 days.The dark avoidance,grasping and autonomous activity were used to observe the learning and memory ability and exercise endurance of mice.The organ index was calculated and the degree of organ damage was observed with HE staining.Results Compared with CON,SCP did not cause the death of mice in 7 days.The physiological reaction,mental state and behavioral activities had no abnormality.There was no significant difference in activity times,standing times,avoidance errors,avoidance latency and forelimb grip strength(P>0.05).There were no significant differences in heart index,liver index,lung index,spleen index,kidney index,brain organ index(P>0.05).Pathological sections showed that the structure of all organs were normal without obvious lesions.Conclusion MTD of SCP is 24 g/kg,SCP had no significant effect on growth,physiological status and behavior of mice.

关 键 词：五味子藤茎 多糖 安全性 小鼠 

分 类 号：R9[医药卫生—药学]