中药材浓缩液在马铃薯脱毒苗培养中的应用  

Application of Concentrated Liquid of Chinese Herbal in The Culture of Virus-free Potato Seedlings

作　　者：赵云彤[1] 解国庆[1] 范书华[1] 王艳[1] 张丽微 华玉晨 时新瑞[1] 董清山[1] ZHAO Yun-tong;XIE Guo-qing;FAN Shu-hua;WANG Yan;ZHANG Li-wei;HUA Yu-chen;SHI Xin-rui;DONG Qing-shan(Mudanjiang Branch,Heilongjiang Academy of Agricultural Sciences,Mudanjiang 157041,China)

机构地区：[1]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041

出　　处：《黑龙江农业科学》2022年第11期41-45,共5页Heilongjiang Agricultural Sciences

基　　金：牡丹江市指导性科技计划项目(SQ2020NS043)。

摘　　要：为改良马铃薯脱毒苗培养基,采用富金和中薯5号马铃薯脱毒苗作为供试材料,将中药材浓缩液应用到马铃薯脱毒苗的培养基中,明确中药材浓缩液对马铃薯脱毒苗生长的影响。结果表明,添加15/1000 mL浓度的中药材浓缩液可以增加马铃薯脱毒苗的根长、叶片数和茎节数,缩短茎节长度,提高脱毒试管苗的鲜重、单瓶结薯数和移栽成活率。与对照相比,马铃薯脱毒苗的根长可增加0.6 cm,叶片数增加0.7~0.8片,茎节数增加1节,茎节长度缩短0.14~0.26 cm,脱毒试管苗单株茎叶鲜重增加0.04~0.06 g,单瓶结薯数增加3~4个。脱毒苗移栽成活率可提高3.0%~4.5%。同时,在脱毒苗炼苗过程中,能有效减少杂菌污染,延长炼苗时间3~4 d。In order to improve the culture medium of potato virus-free seedlings,Fujin and Zhongshu No.5potato virus-free seedlings were used as test materials,and the concentrated liquid of Chinese herbal medicine was applied to the culture medium of potato virus-free seedlings to determine the effect of the concentrated liquid of Chinese herbal medicine on the growth of potato virus-free seedlings.The results showed that the addition of appropriate concentration of Chinese herbal medicine concentrate could increase the root length,leaf number and stem node number of potato virus-free seedlings,and shorten the stem node length.The fresh weight,number of tubers per bottle and the transplanting survival rate of virus-free test-tube seedlings were improved.Compared with the control,the root length of virus-free potato seedlings increased by 0.6cm,the number of leaves increased by 0.7-0.8leaves,the number of stem nodes increased by 1node,the length of stem node was shortened by 0.14-0.26cm,and the fresh weight of stem and leaf per plant of virus-free test tube plantlet increased by 0.04-0.06g,and the number of virus-free mini tubers increased by 3-4tubers per bottle.The survival rate of transplanting could be increased by 3.0%-4.5%.Moreover,after adding the concentrated liquid of Chinese herbal medicine,the pollution of fungi can be reduced and the time of hardening seedlings can be prolonged 3-4days in the process of hardening seedlings.

关 键 词：中药浓缩液 马铃薯脱毒苗 培养基 

分 类 号：S532[农业科学—作物学]