NaCl胁迫对中间偃麦草种子萌发和幼苗生长的影响  

Effects of NaCl Stress on Seed Germination and Seedling Growth of Elytrigia intermedia

作　　者：王建丽[1] 牟林林 尤佳 张冬梅[1] 庄煦 宋雨桐 邸桂俐[1] WANG Jian-li;MU Lin-lin;YOU Jia;ZHANG Dong-mei;ZHUANG Xu;SONG Yu-tong;DI Gui-li(Institute of Forage and Grassland Science,Heilongjiang Academy of Agricultural Science,Harbin 150086,China;College of Life Science and Technology,Harbin Normal University,Harbin 150025,China)

机构地区：[1]黑龙江省农业科学院草业研究所,黑龙江哈尔滨150086 [2]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025

出　　处：《黑龙江农业科学》2022年第11期62-67,共6页Heilongjiang Agricultural Sciences

基　　金：黑龙江省省属科研院所科研业务费(CZKYF2022-1-B012)。

摘　　要：为确定不同中间偃麦草种质对NaCl的耐受值,以5份中间偃麦草材料为研究对象,利用不同浓度NaCl溶液(25,50,100,200,300和400 mmol·L^(-1))模拟盐胁迫,测定种子的发芽率、发芽势、发芽指数、活力指数,相对盐害率,以及幼苗胚根长和胚芽长,并建立相对发芽率与盐胁迫浓度的回归方程。结果表明,随盐胁迫浓度增加,中间偃麦草种子萌发指标呈下降趋势,相对盐害率呈上升趋势,幼苗胚根、胚芽长也呈下降趋势,且盐胁迫对胚根抑制作用大于胚芽。具体表现为低浓度NaCl(25 mmol·L^(-1))胁迫对5份种质的发芽率,发芽指数、活力指数和胚芽长无显著影响,随盐胁迫浓度增加,种子萌发抑制程度加剧。当中度NaCl(200 mmol·L^(-1))胁迫时,除中偃25号外,其他4份材料的发芽率在75%以上,中偃25号种子发芽势为0。高浓度NaCl(300 mmol·L^(-1))胁迫下,5份材料的发芽率、发芽指数大幅度降低,相对盐害率大幅提高,其中中偃3号相对盐害率最高,为82.12%,中偃24号相对盐害率最低,为66.58%。当盐浓度达到400 mmol·L^(-1),所有材料均不萌发,相对盐害率达100%。根据建立的最优一元二次回归方程可知,在种子萌发阶段,种子耐盐性从大到小依次为中偃13号>中偃24号>中偃25号>中偃3号>中偃1号。In order to determine the tolerance value of different Elytrigia intermedia germplasms to NaCl,five samples of Elytrigia intermedia were used to simulate salt stress with different concentrations of NaCl solution(25,50,100,200,300,400 mmol·L^(-1)).The germination rate,germination potential,germination index,vigor index,relative salt injury rate,and radicle length and plumule length of seedlings were measured,and the regression equation between relative germination rate and salt stress concentration was established.The results showed that with the increase of salt stress concentration,the germination index of seeds showed a downward trend,the relative salt damage rate showed an upward trend,and the radicle and embryo length also showed a downward trend,and the inhibition of salt stress on the radicle was greater than that on the embryo.The specific performance was that the low concentration NaCl(25 mmol·L^(-1))stress had no significant effect on the germination rate,germination index,vigor index and e mbryo length of five germplasms.With the increase of salt stress concentration,the inhibition of seed germination increased.Under moderate NaCl(200 mmol·L^(-1))stress,except Zhongyan 25,the germination rate of other four materials were more than 75%,and the germination potential of Zhongyan 25 seed was 0.Under the salt stress of high concentration(300 mmol·L^(-1)),the germination rate and germination index of five materials decreased significantly,and the relative salt damage rate increased significantly.Among them,Zhongyan 3 had the highest relative salt damage rate of 82.12%,and the Zhongyan 24 had lowest relative salt damage rate of 66.58%.When the salt concentration reached 400 mmol·L^(-1),all materials did not germinate,and the relative salt damage rate reached 100%.According to the optimal one variable quadratic regression equation established,the salt tolerance of seeds at the germination stage was in the order of Zhongyan 13>Zhongyan 24>Zhongyan 25>Zhongyan 3>Zhongyan 1.

关 键 词：中间偃麦草 NACL胁迫 种子萌发 幼苗生长 耐盐性 

分 类 号：S512.1[农业科学—作物学]