一株矿区锰氧化细菌的分离鉴定及性能研究  

Isolation and Identification of a Manganese Oxidizing Bacteria in Mining Area

作　　者：李梦玲 张兰 吉瑞麟 彭延华 袁本琦 侯冬梅 邹建平[1] LI Meng-ling;ZHANG Lan;JI Rui-lin;PENG Yan-hua;YUAN Ben-qi;HOU Dong-mei;ZOU Jian-ping(School of Environmental and Chemical Engineering,Nanchang Hangkong University,Nanchang 330063;Jiangxi Fact-seeking Forensic Appraisal Center,Nanchang 330096)

机构地区：[1]南昌航空大学环境与化学工程学院,南昌330063 [2]江西求实司法鉴定中心,南昌330096

出　　处：《南昌航空大学学报（自然科学版）》2022年第3期22-29,81,共9页Journal of Nanchang Hangkong University(Natural Sciences)

基　　金：国家自然科学基金(52200097);江西省教育厅科技项目(DA202202121);南昌航空大学博士启动基金(EA202102234)。

摘　　要：利用微生物修复矿区酸性废水是一种高效、经济且环境友好的方法。本文从湖南七宝山矿区分离得到一株锰氧化细菌,经生理生化鉴定及16S rRNA扩增子测序分析表明:该菌为土壤芽孢杆菌属Brevibacillus agri,革兰氏阳性菌,将其命名为Brevibacillus agri NC-1。在500 mg/L的锰离子浓度下,经单因素实验优化获得该菌的最佳培养条件为:pH=7,温度30℃,接种量3%,转速150 r/min,且培养7 d后Brevibacillus agri NC-1对锰离子的去除率可达98%。因此该菌具有较强的除锰能力,可作为矿区酸性废水中锰污染修复的功能菌种。Bioremediation is an efficient,economical and environmentally friendly method to treat acid mine drainage.In this paper,a manganese-oxidization bacterium was isolated from Qibaoshan mining area.The physiological and biochemical identification and 16S rRNA amplicome sequencing analysis showed that the bacterium was Gram-positive Bacillus Brevibacillus Agri,which was named Brevibacillus agri NC-1.The optimal culture conditions of Brevibacillus agri NC-1 were as follows:pH=7,temperature 30℃,inoculum size 3%,speed 150 r/min.The removal rate of manganese ions from Brevibacillus agri NC-1 reached 98%after cultured 7 days(initial manganese ion concentration:500mg/L).Therefore,this bacterium had strong ability to remove manganese and can be used as a functional strain for acid mine drainage remediation.

关 键 词：锰氧化细菌 矿山酸性废水 分离 鉴定 Mn2+去除 

分 类 号：Q93[生物学—微生物学]