硫酸-甲醇衍生化对烟草中乙酰丙酸质量分数测定的影响  

Effect of derivatization with sulfuric acid and methanol on quantification of levulinic acid in tobacco

作　　者：刘瑞红 谢复炜[1] 王晓瑜[1] 潘立宁[1] 余晶晶[1] 刘克建[1] 陈满堂 孙学辉[1] 黄龙[2] LIU Ruihong;XIE Fuwei;WANG Xiaoyu;PAN Lining;YU Jingjing;LIU Kejian;CHEN Mantang;SUN Xuehui;HUANG Long(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;China Tobacco Hubei Industrial Co.,Ltd.,Wuhan 430048,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]湖北中烟工业有限责任公司,武汉市430048

出　　处：《烟草科技》2022年第10期53-61,共9页Tobacco Science & Technology

基　　金：国家烟草专卖局科技重点研发项目“烤烟烟叶风格与质量的化学表征组学研究”(110202002003);国家烟草专卖局创新平台科研活动稳定支持专项经费资助(312021AW0420)。

摘　　要：为考察采用硫酸-甲醇衍生化方法测定烟草中乙酰丙酸质量分数的可靠性,分析了硫酸-甲醇衍生化时间和温度对烟草中乙酰丙酸质量分数测定的影响,探索了5种糖类成分在硫酸-甲醇衍生化条件下是否产生乙酰丙酸甲酯,比较了263个烟草实际样品中果糖和蔗糖质量分数与乙酰丙酸质量分数的关系。结果表明:①硫酸-甲醇衍生化时间和温度对烟草样品甲酯化产物中乙酰丙酸甲酯响应的影响不同于其他有机酸甲酯,且这种不同与烟草基质有关;②果糖和蔗糖在硫酸-甲醇衍生化条件下能产生乙酰丙酸甲酯,而葡萄糖、麦芽糖和肌醇在衍生化过程中不产生乙酰丙酸甲酯;③蔗糖在硫酸-甲醇衍生化条件下产生的乙酰丙酸甲酯的质量仅为相同质量果糖产生的1/2;④利用硫酸-甲醇衍生化法测定得到的烟草中乙酰丙酸的质量分数受烟草中果糖和蔗糖的影响。To investigate the effect of derivatization with sulfuric acid and methanol on measuring the mass fraction of levulinic acid in tobacco,the influences of the derivation time and temperature were analyzed.In addition,five sugars were investigated under the conditions of derivatization on possible formation of levulinic acid methyl ester.Relationships between the mass fractions of fructose and sucrose and the mass fraction of levulinic acid in 263 tobacco samples were analyzed and compared.The results showed that:1)The effects of the derivatization time and temperature on the response of levulinic acid methyl ester were different from the response of other organic acids methyl ester in methyl esterified tobacco sample,and those differences were related to the tobacco matrix.2)Fructose and sucrose could produce levulinic acid methyl ester under the conditions of derivatization,while glucose,maltose and inositol could not.3)The levulinic acid methyl ester mass produced by sucrose under the conditions of derivatization was only half of that by fructose at the same mass.4)The mass fraction of levulinic acid in tobacco detected by the proposed derivatization method with sulfuric acid and methanol was affected by the fructose and sucrose levels in tobacco.

关 键 词：烟草 硫酸-甲醇衍生化 乙酰丙酸 衍生化时间 衍生化温度 糖 

分 类 号：TS411.1[农业科学—烟草工业]