饲粮中添加硒对母羊发情周期生殖激素的影响  

Effects of dietary selenium supplementation on reproductive hormones in ewes during estrous cycle

作　　者：黎明[1] 扎拉嘎 满达[1] 唐和斯 李云鹏 双金[1] LI Ming;Zhalaga;Manda;TANG Hesi;Li Yunpeng;Shuangjin(Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Shengmu High Tech.Animal Husbandry Co.,Ltd.,,Hohhot 010010,China)

机构地区：[1]内蒙古农业大学,呼和浩特010018 [2]内蒙古圣牧高科牧业有限公司,呼和浩特010010

出　　处：《黑龙江动物繁殖》2022年第5期12-16,共5页Heilongjiang journal of animal reproduction

摘　　要：为了探讨饲粮中添加活性硒对母羊发情周期生殖激素的影响,试验将2~3岁的20只健康蒙古羊随机分为2组,对照组饲喂基础饲粮,试验组饲喂含有1%活性硒的饲粮。试验期为36d,其中预试期为15d、正试期为21d。在预试期5d后,给每只母羊肌内注射1mL氯前列腺烯醇(PG),隔8d再注射PG1mL,6d后给每只羊安装阴道栓,11d后撤阴道栓并肌内注射孕马血清促性腺激素(PMSG)400IU。每组随机选取6只母羊在撤栓前1天及发情周期第1,10,20天采血,分离血清并用放射免疫分析法测定血清雌激素(E_(2))、孕酮(P)、黄体生成激素(LH)和卵泡生成激素(FSH)含量。结果表明:与对照组相比,试验组母羊血清E_(2)、P、LH和FSH含量有提高的趋势;母羊血清E_(2)含量在发情初期最高(P<0.05),P含量由发情周期第10天左右进入高位态势(P<0.05),血清LH和FSH含量也是初期高、而后进入下降态势。说明饲粮硒能够促进母羊繁殖相关激素的分泌。In order to investigate the effect of dietary active selenium supplementation on reproductive hormones of ewes in estrus cycle,20 Mongolian ewes at 2~3 yr old,with good body condition,randomly divided into two groups.The control group fed with basic diet,while the experimental group fed with diet containing 1%active selenium.The trial period was 36 d with the pre-trial period of 15 d and the formal trial period of 21 d.After 5 d of pre-feeding,every ewe was given intramuscular injection of 1 mLof chloroprostanol(PG),and then injected with 1 mLof PG at the day 13 of trial.Every ewe was given vaginal suppository at the day 19 of trial;The vaginal suppository was withdrawn and 400 IU of PMSG was injected intramuscularly at the day 30 of the trial.Six ewes in each group were randomly selected to collect the serum samples at the day before the day of embolectomy,1 st d,10 th d and 20 th d of estrus cycle,and the contents of serum E_(2),P_(4),LH and FSH were determined by radioimmunoassay.The results showed that compared with the control group,the contents of E_(2),P,LH and FSH in serum of ewes in experimental group were increased.During the oestrus cycle,the content of serum E_(2)was found the highest at the beginning of oestrus(P<0.05),the content of serum progesterone was at high level at the day 10 of oestrus cycle,the content of serum LH and FSH was at high level in the beginning stage of cycle,and then decreased.It could be concluded that dietary active selenium supplementation would promote the secretion of reproductive hormones in ewes.

关 键 词：活性硒 母羊 雌激素(E2) 孕酮(P) 黄体生成激素(LH) 卵泡生成激素(FSH) 

分 类 号：S826.5[农业科学—畜牧学] S816.7[农业科学—畜牧兽医]