三维细胞培养模型在人乳头瘤病毒相关癌症中的研究进展  

Research advances in three-dimensional cell culture models in human papillomavirus-associated cancers

作　　者：陈昕煜 于大海[2] Chen Xinyu;Yu Dahai(College&Hospital of Stomatology,Guangxi Medical University,Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction,Guangxi Clinical Research Center for Craniofacial Deformity,Guangxi Health Commission Key laboratory of prevention and treatment for oral infectious diseases,Nanning 530021,China;Department of Stomatolog,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学口腔医学院/附属口腔医院,广西口腔颌面修复与重建研究自治区级重点实验室,广西颅颌面畸形临床医学研究中心,广西壮族自治区卫生健康委员会,口腔感染性疾病防治重点实验室,南宁530021 [2]广西医科大学第一附属医院口腔科,南宁530021

出　　处：《广西医科大学学报》2022年第10期1672-1676,共5页Journal of Guangxi Medical University

基　　金：广西自然科学基金重点项目(No.2016GXNSFDA380002)。

摘　　要：人乳头瘤病毒(HPV)是一种常见的传染性病原体,目前与HPV感染相关的宫颈、肛门和口咽癌发病率持续增高,已经成为当下全球癌症防治的热点。由于HPV的生命周期对多层上皮宿主细胞分化程序的依赖性以及种属特异性,使得建立一个适合的HPV体外感染模型十分困难。随着三维(3D)细胞培养系统的出现,克服了这一障碍,这一系统完整地再现了上皮细胞的分化,为完成HPV的生命周期提供了一个良好的生存环境,是更好地研究HPV相关癌症的发生发展以及治疗药物的体外模型。本文将从HPV特性的角度分析3D细胞培养在HPV相关癌症研究中的优势,介绍适用的3D细胞培养方式,总结分析3D细胞培养在HPV相关癌症研究中应用及展望,为优化研究HPV相关癌症的3D细胞培养模型提供理论依据。Human papillomavirus(HPV)is a common infectious pathogen,and the incidence of cervical,anal and oropharyngeal cancers associated with HPV infection continues to increase and has become a current global hotspot for cancer prevention and treatment.The dependence of the HPV life cycle on the differentiation program of the multilayer epithelial host cells and its species specificity make it difficult to establish a suitable HPV infection model in vitro.This obstacle has been overcome with the advent of the three-dimensional(3D)cell culture system,a system that completely recapitulates epithelial cell differentiation,provides a good survival environment for completing the HPV life cycle,and is a better in vitro model for studying the development of HPV-associated cancers as well as therapeutic agents.This paper analyzes the advantages of 3D cell culture in HPV-associated cancer research from the perspective of HPV characteristics,introduces applicable 3D cell culture methods,summarizes as well as analyzes the application and outlook of 3D cell culture in HPV-associated cancer research so as to provide a theoretical basis for optimizing 3D cell culture models for studying HPV-associated cancers.

关 键 词：三维细胞培养 人乳头瘤病毒(HPV) 癌症 

分 类 号：R446.11[医药卫生—诊断学]