基于单细胞转录组测序技术对鄂尔多斯细毛羊次级毛囊形态发生诱导期的研究  

Induction of Secondary Hair Follicle Morphogenesis in Ordos Fine Wool SheepBased on Single-cell Transcriptome Sequencing Technology

作　　者：赫雪 谷英 斯登丹巴 德德玛 窦骁宁 吴怡 雷志惠 潘和平[1] 岳耀敬 HE Xue;GU Ying;SIDENG Danba;DE Dema;DOU Xiaoning;WU Yi;LEI Zhihui;PAN Heping;YUE Yaojing(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;Erdos Institute of Animal Husbandry Science,Erdos 017100,China)

机构地区：[1]西北民族大学生命科学与工程学院,兰州730030 [2]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [3]鄂尔多斯市农牧业科学研究院,鄂尔多斯017100

出　　处：《中国畜牧兽医》2022年第11期4296-4306,共11页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划“绵羊高效育种与繁殖关键技术研发”(2021YFD1300901);中国农业科学院科技创新工程重大科研任务“优质高效肉羊新品种培育与产业化”(CAAS-ZDRW202106);国家绒毛用羊产业技术体系育种技术与方法岗位(CARS-39-02);内蒙古自治区科技计划项目“鄂尔多斯细毛羊细型新品种培育”(2021GG0320)。

摘　　要：【目的】试验旨在解析鄂尔多斯细毛羊胚胎期次级毛囊诱导期形态发生过程中主要细胞类型的分子特征和分化过程。【方法】对采集的3只鄂尔多斯细毛羊(胎龄87 d)体侧部(肩胛骨后缘处)的皮肤样本进行HE染色,鉴定毛囊的发育时期;部分皮肤样本经过混样后进行单细胞转录组测序(scRNA-Seq),应用t-分布随机近邻嵌入(tSNE)分析细胞簇,分别使用胶原Ⅰ型α1链(Col1a1)和角蛋白15(Krt15)鉴定真皮谱系细胞和表皮谱系细胞,并使用皮肤组织不同细胞的标记基因进行细胞类型分析;对测序数据进行拟时序分析,探究其分化过程中差异基因的表达;通过GO功能富集分析进一步验证基因的功能。【结果】HE染色结果发现,鄂尔多斯细毛羊在胎龄87 d处于次级毛囊诱导期。通过scRNA-Seq在胎龄87 d的细毛羊体侧部皮肤细胞样品中获得10603个细胞和18704个基因的scRNA-Seq数据可用于下游分析。tSNE分析发现,皮肤组织中共有15个细胞簇;Col1a 1和Krt 15标记基因鉴定表明,真皮细胞和表皮细胞具有高度异质性。拟时序分析构建毛囊形态发生过程中真皮/表皮细胞谱系细胞的分化轨迹和基因动态表达图谱表明,在真皮谱系细胞由成纤维细胞(Fb)向成熟真皮聚凝物(DC)的分化过程中,多个不同阶段的标记基因FST重组蛋白(Fst)、抑制素亚基βα(Inhba)和转录阻遏物GATA结合1(Trps1)均在拟时序轨道上特异性表达,并富集了Wnt、Noggin和骨形态发生蛋白(BMP)等与毛囊形态发生相关的信号通路;在表皮谱系细胞分化过程中,基质和毛囊间表皮(IFE)的标记基因角蛋白10(Krt10)、同源基因C13(HOXC13)和音猬因子信号(SHH)在表皮谱系细胞拟时序轨道的2个分支上均特异性表达,且富集在细胞增殖和细胞黏附等相关通路。【结论】在细毛羊次级毛囊诱导期,真皮谱系细胞由成纤维细胞分化至真皮聚凝物,表皮谱系细胞处于基质和毛囊间表�【Objective】The purpose of this experiment was to analyze the molecular characteristics and differentiation process of main cell types in the morphogenesis of the secondary hair follicles in Erdos fine wool sheep during the embryonic stage.【Method】HE staining was performed on skin samples from the lateral part(posterior edge of scapula)of 87 fetal age of 3 Ordos fine wool sheep to identify the development period of hair follicles.Single-cell transcriptome sequence(scRNA-Seq)was performed on some skin samples after mixing.Cell clusters were analyzed by t-distributed stochastic Neigh or embedding(tSNE),and dermal lineage cells and epidermal lineage cells were identified by Col1a 1 and Krt 15 genes,respectively.The marker genes of different skin cells were used for cell type analysis.The sequence data were analyzed to explore the expression of differential genes in the process of differentiation.The function of the gene was further verified by GO function enrichment analysis.【Result】HE staining results showed that Ordos fine wool sheep were in the secondary hair follicle induction stage at 87 days of gestational age.10603 cells and 18704 genes were obtained by scRNA-Seq in skin cell samples from the body side of fine wool sheep at 87 days of gestational age could be used for downstream analysis.Based on tSNE analysis,15 cell clusters were found in scRNA-Seq data.The identification of Col1a 1 and Krt 15 marker genes showed that dermal and epidermal cells were highly heterogeneous.The differentiation trajectories and dynamic gene expression profiles of dermal/epidermal cell lineage in the process of hair follicle morphogenesis were constructed by quasi-sequential analysis,during the differentiation of dermal lineate cells from fibroblast(Fb)to mature dermal condensates(DC),several different stage marker genes FST recombinant protein(Fst),inhibin subunitβα(Inhba)and transcriptional repressor GATA binding 1(Trps1)were specifically expressed on the quasisequential track,and Wnt,Noggin,bone morphogenetic prot

关 键 词：鄂尔多斯细毛羊 次级毛囊 单细胞转录组测序(scRNA-Seq) 毛囊发育 

分 类 号：S826.91[农业科学—畜牧学]