基于网络药理学探究乌梅散加减方治疗猪传染性胃肠炎的作用机制  

Exploration on the Mechanism of Modified Wumei Powder in the Treatment of Porcine Transmissible Gastroenteritis Based on Network Pharmacology

作　　者：樊俊洋 吴文义 张云天 金钺 杨明凡 张红英[1,2,3] FAN Junyang;WU Wenyi;ZHANG Yuntian;JIN Yue;YANG Mingfan;ZHANG Hongying(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China;Henan Key Laboratory of Animal Food Safety,Zhengzhou 450002,China;Key Laboratory of Traditional Chinese Veterinary Drug Development and Evaluation,Zhengzhou 450002,China)

机构地区：[1]河南农业大学动物医学院,郑州450002 [2]河南省动物性食品安全重点实验室,郑州450002 [3]河南省郑州市中兽药创制与评价重点实验室,郑州450002

出　　处：《中国畜牧兽医》2022年第11期4447-4456,共10页China Animal Husbandry & Veterinary Medicine

基　　金：河南省重点研发项目(221111111300);国家自然科学基金项目(30900472)。

摘　　要：【目的】借助网络药理学手段探究乌梅散加减方治疗猪传染性胃肠炎(TGE)的作用机制。【方法】利用中药系统药理学数据库与分析平台(TCMSP)、Swiss TargetPrediction获取乌梅散加减方的活性成分与作用靶点,应用公共比较毒理基因组学数据库(CTD)获取TGE的相关靶点,使用STRING数据库绘制乌梅散加减方与TGE交集靶点的蛋白互作(PPI)网络图,利用DAVID对交集靶点进行GO功能和KEGG通路富集分析,探究乌梅散加减方治疗TGE的作用机制。【结果】从乌梅散加减方中共筛选到槲皮素、山柰酚、芒柄花素等125种活性成分和JUN原癌基因(JUN)、肿瘤坏死因子(TNF)、信号传导与转录激活因子3(STAT3)等58个作用靶点。GO功能富集分析显示,共得到生物过程138个条目,涉及免疫反应、血管内皮因子生成、RNA聚合酶Ⅱ启动子对pri-miRNA转录的正调控等;细胞组分13个条目,涉及胞外区、细胞外空间、膜筏等;分子功能32个条目,涉及细胞因子活性、生长因子活性、白细胞介素2(IL2)受体活性等。KEGG通路富集分析显示,共得到135条信号通路,其中IL17信号通路、Th17细胞分化、TNF信号通路、HIF-1信号通路是乌梅散加减方治疗TGE的主要信号通路。【结论】乌梅散加减方主要通过槲皮素、山柰酚、芒柄花素等多个活性成分调控JUN、TNF、STAT3等靶点,通过参与IL17信号通路、Th17细胞分化等治疗TGE。【Objective】 The purpose of this study was to explore the mechanism of modified Wumei powder in the treatment of porcine transmissible gastroenteritis(TGE) by network pharmacology.【Method】 The active components and action targets of modified Wumei powder were obtained using Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP) and Swiss TargetPrediction, and the related targets of TGE were obtained using the Public Comparative Toxicology Genomics Database(CTD).The protein interaction(PPI) network diagram of the intersection targets of modified Wumei powder and TGE was drawn using STRING database.The GO function and KEGG pathway enrichment analysis of the intersection targets were performed by DAVID,so as to explore the main action mechanism of modified Wumei powder in the treatment of TGE.【Result】 A total of 125 active components such as quercetin, kaempferol and formononetin and 58 action targets such as JUN,TNF and STAT3 were screened from modified Wumei powder.GO functional enrichment analysis showed that a total of 138 items of biological process were obtained, including immunoreaction, generation of vascular endothelial factors, and positive regulation of pri-miRNA transcription by the RNA polymerase Ⅱ promoter.Cellular component was 13 items, involving extracellular region, extracellular space, membrane raft, etc.Molecular function was 32 items, involving cytokine activity, growth factor activity, and interleukin 2(IL2) receptor activity.Enrichment analysis of KEGG pathway revealed that a total of 135 signaling pathways were identified, including IL17 signaling pathway, Th17 cell differentiation, TNF signaling pathway, and HIF-1 signaling pathway, which were the main pathways of modified Wumei powder in the treatment of TGE.【Conclusion】 The results above indicated that modified Wumei powder mainly regulated JUN,TNF and STAT3 targets by multiple active components such as quercetin, kaempferol and formononetin, and treated TGE by participating in IL17 signalin

关 键 词：乌梅散加减方 猪 传染性胃肠炎(TGE) 网络药理学 

分 类 号：S853.74[农业科学—临床兽医学]