水稻不育系湘陵628S不同组合感光性差异的遗传解析  

Genetic analysis of photosensitivity divergence among hybrids derived from rice sterile line Xiangling 628S

作　　者：陈赛华 彭盛 尤仪雯 张路遥 王凯 薛明 杨远柱 万建民 CHEN Sai-Hua;PENG Sheng;YOU Yi-Wen;ZHANG Lu-Yao;WANG Kai;XUE Ming;YANG Yuan-Zhu;WAN Jian-Min(Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology,Agricultural College of Yangzhou University,Yangzhou 225009,Jiangsu,China;State Key Laboratory for Crop Genetics and Germplasm Enhancement/Jiangsu Plant Gene Engineering Research Center/Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;Key Laboratory of Southern Rice Innovation&Improvement,Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding/Yuan Longping High-Tech Agriculture Co.,Ltd.,Changsha 410128,Hunan,China)

机构地区：[1]江苏省作物基因组学和分子育种重点实验室/植物功能基因组学教育部重点实验室/江苏省作物遗传生理重点实验室,扬州大学农学院,江苏扬州225009 [2]南京农业大学/作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心,江苏南京210095 [3]袁隆平农业高科技股份有限公司/农业农村部南方水稻品种创制重点实验室/抗病虫水稻育种湖南省工程实验室,湖南长沙410128

出　　处：《作物学报》2023年第2期332-342,共11页Acta Agronomica Sinica

基　　金：江苏省农业自主创新基金项目(基于qTT12的水稻耐高温种质资源创制)(CX(21)3100);湖南省科技创新计划项目(2021NK1001)资助。

摘　　要：湘陵628S是优质矮秆抗倒两系早稻不育系,所配组合米质优,且适宜轻简化、规模化和机械化种植,目前已有40个“陵两优”系列品种通过国家或省级审定,并大面积推广应用。测配中发现,湘陵628S与绝大多数中晚稻亲本配组感光性强,在长江流域不能正常抽穗,限制了其育种应用。为了探究湘陵628S及不同杂交组合感光性差异的遗传机制,本研究结合等位性测验与基因型分析,对湘陵628S及恢复系的关键感光基因进行分析。结果发现,湘陵628S携带有隐性等位基因e_(1)和Se-1^(e),显性等位基因Hd5^(k)、E_(2)、E_(3)和EF-1^(t),总体表现出弱感光性。恢复系携带的E_(1)(Ghd7)基因决定了不同组合的感光性强弱,所携带的Se-1(Hd1)和Hd5(DTH8)基因与感光性没有直接相关性。据此,我们开发了2个分子标记用于恢复系E_(1)、Se-1等位基因型的分子鉴定,以加快弱/不感光杂交组合的选育。本研究为湘陵628S及其他不育系在杂交育种上的利用提供了重要的理论和技术指导。Xiangling 628S is a high quality thermosensitive male sterile line of two-line hybrid rice,whose hybrids have high-quality rice and is suit for light-cultivation,large-scale,and mechanized planting,and 40 new hybrid rice varieties have been approved and widely used.However,its hybrids had strong photosensitivity when it crossed with a large majority of mid-late cultivars,and headed late or even not heading under long-day(LD)conditions,which limited its application in the Yangtze River area.To explore the genetic mechanism of photosensitivity divergence among different hybrids,several key photosensitive loci in Xiangling 628S and its restorer lines were analyzed by allelism tests combined with sequence analysis.The results showed that Xiangling 628S had recessive e_(1)e_(1),Se-1^(e)Se-1^(e) and dominant Hd5^(k)Hd5^(k),E_(2)E_(2),E_(3)E_(3),and EF-1^(t)EF-1^(t),showing weak photosensitivity.The photosensitivity divergence in different hybrids was closely correlated with E_(1)(Ghd7)locus in restorer lines,but not with Se-1(Hd1)and Hd5(DTH8).Finally,we developed two functional molecular markers for genetic identification of E1 and Se-1 loci in restorer lines,which would speed up the selection of weak/non-sensitive hybrids of Xiangling 628S.Our study provides important theoretical guidance and technical supports for the utilization of Xiangling 628S in breeding,as well as hybrid breeding with other sterile lines.

关 键 词：水稻 不育系 感光性 基因 分子标记 

分 类 号：S511[农业科学—作物学]