丝素蛋白/氧化石墨烯纳米纤维膜的制备与性能  

A Study on the Fabrication and the Properties of the Silk Fibroin/Graphene Oxide Nanofibrous Membrane

作　　者：程若冰 何一强 唐嘉俊 杨雨欣 刘欣玥 罗袁俊 张葵花[2] Cheng Ruobing;He Yiqiang;Tang Jiajun;Yang Yuxin;Liu Xingyue;Luo Yuanjun;Zhang Kuihua(Analytical and Testing Center,Jiaxing University,Jiaxing,Zhejiang 314001;College of Materials and Textile Engineering,Jiaxing University,Jiaxing,Zhejiang 314001)

机构地区：[1]嘉兴学院分析测试中心,浙江嘉兴314001 [2]嘉兴学院材料与纺织工程学院,浙江嘉兴314001

出　　处：《嘉兴学院学报》2022年第6期54-58,共5页Journal of Jiaxing University

基　　金：浙江省自然科学基金项目(LY20C100003);嘉兴市公益项目(2021AY10062);国家级大学生创新创业训练计划项目(202110354003);嘉兴学院重点SRT项目(CD8517211221)。

摘　　要：以水为溶剂,采用静电纺丝的方法制备丝素蛋白(SF)/氧化石墨烯(GO)共混纳米纤维膜,并用75%(V/V)乙醇蒸汽对其处理.用扫描电镜(SEM)和衰减全反射红外光谱(ATR-FTIR)表征乙醇蒸汽处理前后SF/GO共混纳米纤维的形貌和化学结构.SEM的测试结果表明,随着GO含量的提高,纳米纤维的直径略有减小,经75%(V/V)乙醇蒸汽处理后,SF/GO共混纳米纤维的形貌基本不变.ATR-FTIR的测试结果表明,GO不能使SF的构象发生转变,而经75%(V/V)乙醇蒸汽处理后,SF的构象由溶于水的无规卷曲结构转变成不溶于水的β-折叠结构.吸水率和细胞增殖实验结果显示,SF/GO共混纳米纤维膜相较于纯SF纳米纤维膜具有更优异的亲水性和细胞相容性,因此SF/GO共混纳米纤维膜有望成为理想的组织再生支架.The silk fibroin(SF)/graphene oxide(GO)blended nanofibrous membrane is fabricated by electrospinning with water as the solvent and treated with 75%(V/V)ethanol steam.The morphology and chemical structure of the SF/GO blended nanofiber before and after the treatment are characterized by scanning electron microscopy(SEM)and attenuation total reflection Fourier transform infrared spectroscopy(ATR-FTIR).The SEM test results show that the diameter of the SF/GO blended nanofiber decreases slightly with the increase of the GO content,and the morphology of the SF/GO blended nanofiber almost remains unchanged after the treatment.ATR-FTIR spectra show that GO could not change the conformation of SF but after the treatment,the conformation of SF changes from the water-soluble random curl structure to the water-insolubleβ-sheet structure.The results of the water absorption and cell proliferation test demonstrate that the SF/GO blended nanofibrous membrane has better hydrophilicity and cytocompatibility than the pure SF nanofibrous membrane.Therefore,the SF/GO blended nanofibrous membrane is expected to become an ideal scaffold for tissue regeneration.

关 键 词：氧化石墨烯 丝素蛋白 纳米纤维 细胞相容性 

分 类 号：TB383[一般工业技术—材料科学与工程]