Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C18 column/time-of-flight mass spectrometry system  

作　　者：Xinyi Chai Yanqiu Gu Lei Lv Chun Chen Fei Feng Yan Cao Yue Liu Zhenyu Zhu Zhanying Hong Yifeng Chai Xiaofei Chen 

机构地区：[1]School of Pharmacy,Shanghai Key Laboratory for Pharmaceutical Metabolite Research,Naval Medical University(Second Military Medical University),Shanghai 200433,China [2]Department of Pharmacy,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,201999,China [3]Department of Pharmacy,Shanghai Eastern Hepatobiliary Surgery Hospital,Naval Medical University(Second Military Medical University),Shanghai,200438,China

出　　处：《Journal of Pharmaceutical Analysis》2022年第5期725-732,共8页药物分析学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.:82073814,81973291,82122066,and 82003909);the Rising-Star Program of Shanghai Science and Technology Committee(Grant No.:19QA1411500).

摘　　要：Astragali Radix(AR)is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer(NK)cells.However,owing to the complexity of its composition,the specific active ingredients in AR that act on NK cells are not clear yet.Cell membrane chromatography(CMC)is mainly used to screen the active ingredients in a complex system of herbal medicines.In this study,a new comprehensive two-dimensional(2D)NK-92MI CMC/C18 column/time-of-flight mass spectrometry(TOFMS)system was established to screen for potential NK cell activators.To obtain a higher column efficiency,3-mercaptopropyltrimethoxysilane-modified silica was synthesized to prepare the NK-92MI CMC column.In total,nine components in AR were screened from this system,which could be washed out from the NK-92MI/CMC column after 10 min,and they showed good affinity for NK-92MI/CMC column.Two representative active compounds of AR,isoastragaloside Ⅰ and astragaloside IV,promoted the killing effect of NK cells on K562 cells in a dose-dependent manner.It can thus suggest that isoastragaloside Ⅰ and astragaloside Ⅳ are the main immunomodulatory components of AR.This comprehensive 2D NK-92MI CMC analytical system is a practical method for screening immune cell activators from other herbal medicines with immunomodulatory effects.

关 键 词：NK cell activators Cell membrane chromatography Immunomodulatory herbal medicine Covalent bonding 

分 类 号：R284.1[医药卫生—中药学]