Caenorhabditis elegans deep lipidome profiling by using integrative mass spectrometry acquisitions reveals significantly altered lipid networks  

作　　者：Nguyen Hoang Anh Young Cheol Yoon Young Jin Min Nguyen Phuoc Long Cheol Woon Jung Sun Jo Kim Suk Won Kim Eun Goo Lee Daijie Wang Xiao Wang Sung Won Kwon 

机构地区：[1]College of Pharmacy,Seoul National University,Seoul,08826,Republic of Korea [2]School of Pharmaceutical Sciences,Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan,250014,China [3]Biological Engineering Technology Innovation Center of Shandong Province,Heze Branch of Qilu University of Technology(Shandong Academy of Sciences),Heze,Shandong,274000,China

出　　处：《Journal of Pharmaceutical Analysis》2022年第5期743-754,共12页药物分析学报（英文版）

基　　金：supported by the National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)(Grant Nos.:NRF-2018R1A5A2024425,NRF-2012M3A9C4048796,and NRF-2021R1I1A4A01057387);funded by the National Institutes of Health Office of Research Infrastructure Programs(Grant No.:P40 OD010440);supported by Plant Genomics and Breeding Institute at Seoul National University.

摘　　要：Lipidomics coverage improvement is essential for functional lipid and pathway construction.A powerful approach to discovering organism lipidome is to combine various data acquisitions,such as full scan mass spectrometry(full MS),data-dependent acquisition(DDA),and data-independent acquisition(DIA).Caenorhabditis elegans(C.elegans)is a useful model for discovering toxic-induced metabolism,highthroughput drug screening,and a variety of human disease pathways.To determine the lipidome of C.elegans and investigate lipid disruption from the molecular level to the system biology level,we used integrative data acquisition.The methyl-tert-butyl ether method was used to extract L4 stage C.elegans after exposure to triclosan(TCS),perfluorooctanoic acid,and nanopolystyrene(nPS).Full MS,DDA,and DIA integrations were performed to comprehensively profile the C.elegans lipidome by Q-Exactive Plus MS.All annotated lipids were then analyzed using lipid ontology and pathway analysis.We annotated up to 940 lipids from 20 lipid classes involved in various functions and pathways.The biological investigations revealed that when C.elegans were exposed to nPS,lipid droplets were disrupted,whereas plasma membrane-functionalized lipids were likely to be changed in the TCS treatment group.The nPS treatment caused a significant disruption in lipid storage.Triacylglycerol,glycerophospholipid,and ether class lipids were those primarily hindered by toxicants.Finally,toxicant exposure frequently involved numerous lipid-related pathways,including the phosphoinositide 3-kinase/protein kinase B pathway.In conclusion,an integrative data acquisition strategy was used to characterize the C.elegans lipidome,providing valuable biological insights into hypothesis generation and validation.

关 键 词：Caenorhabditis elegans LIPIDOMICS Data-dependent acquisition Data-independent acquisition 

分 类 号：O657.7[理学—分析化学]