Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography  

作　　者：Qiqin Wang Lingjue Sun Huihui Wu Ning Deng Xianglong Zhao Jingwei Zhou Tingting Zhang Hai Han Zhengjin Jiang 

机构地区：[1]Institute of Pharmaceutical Analysis,College of Pharmacy,Jinan University,Guangzhou,510632,China [2]Anhui Prevention and Treatment Center for Occupational Disease,Anhui No.2 Provincial People's Hospital,Hefei,230041,China [3]Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education,Lab of Biochemistry,College of Chemistry,Xiangtan University,Xiangtan,Hunan,411105,China [4]Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research,Jinan University,Guangzhou,510632,China

出　　处：《Journal of Pharmaceutical Analysis》2022年第5期783-790,共8页药物分析学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.:82173773 and 82073806);the Natural Science Foundation of Guangdong Province,China(Grant Nos.:2020A1515010569 and 2021A0505030039);Science and Technology Program of Guangzhou,China(Grant No.:202102020729).

摘　　要：Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction chromatography.In this study,a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-1-propanesulfonate(SBVI)with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic system.Notably,the duration for the preparation of this novel monolith was as little as 5 min,which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated copolymerization.Moreover,these monoliths showed good morphology,permeability,porosity(62.4%),mechanical strength(over 15 MPa),column efficiency(51,230 plates/m),and reproducibility(relative standard deviations for all analytes were lower than 4.6%).Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based monolith.In particular,the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein adsorption.These excellent features seem to favor its application in bioanalysis.Therefore,the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex samples.In this study,we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.

关 键 词：Zwitterionic monolith SULFOBETAINE Photo-initiated copolymerization Hydrophilic interaction chromatography Complex sample 

分 类 号：O652.63[理学—分析化学] TQ460.1[理学—化学]