养殖池塘黄河鲤肠道硫酸盐还原菌群落特征解析  

Community structure analysis of intestinal sulfate-reduction bacteria of Cyprinus carpio L.in aquaculture pond

作　　者：马丽美 蔡慧敏 梁晓敏 游富 江爱霞 杨国坤 张新党 常绪路 张艳敏 孟晓林 MA Li-mei;CAI Hui-min;LIANG Xiao-min;YOU Fu;JIANG Ai-xia;YANG Guo-kun;ZHANG Xin-dang;CHANG Xu-lu;ZHANG Yan-min;MENG Xiao-lin(College of Fisheries,Henan Normal University/Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,Henan Normal University,Xinxiang 453007,Henan,China)

机构地区：[1]河南师范大学水产学院/河南省水产动物养殖工程技术研究中心,河南新乡453007

出　　处：《淡水渔业》2022年第6期3-9,共7页Freshwater Fisheries

基　　金：河南省高等学校重点科研项目(23B240001);国家自然科学基金(U1904118)。

摘　　要：为探究鱼类肠道硫酸盐还原菌(sulfate-reduction bacteria,简称SRB)的群落特征,本研究选用黄河鲤(Cyprinus carpio L.)为研究对象,通过SRB功能基因(dsrB)高通量测序技术,对黄河鲤前、中和后肠SRB功能群进行检测。研究结果显示:黄河鲤肠道SRB主要分布在中肠和后肠,中肠SRB主要包括变形菌门(Proteobacteria)的脱硫球茎菌属(Desulfobulbus)、脱硫弧菌属(Desulfovibrio)、互营杆菌属(Syntrophobacter),厚壁菌门(Firmicutes)的脱亚硫酸菌属(Desulfitobacterium)和绿弯菌门(Chloroflexi)的暖绳菌属(Caldilinea);后肠SRB主要分布在变形菌门的脱硫球茎菌属、脱硫弧菌属、脱硫菌属(Desulfotalea)、互营杆菌属、脱硫念珠菌属(Desulfomonile)和鞘氨醇单胞菌属(Sphingomonas)。依据高通量测序结果的FAPROTAX(Functional Annotation of Prokaryotic Taxa)数据库代谢功能预测显示,黄河鲤肠道SRB主要参与硫化合物呼吸、硫酸盐呼吸和化能异养功能。Cyprinus carpio L.was selected as the research object to explore the intestinal Sulfate reducing bacteria(SRB) of fishThe SRB functional groups in the foregut, midgut and hindgut of C.carpio L.were detected by high throughput sequencing of SRB functional gene(dsrB).The results showed that the intestinal SRB of Cyprinus carpio L.was mainly distributed in the midgut and hindgut.The SRB of midgut were mainly Desulfobulbus,Desulfovibrio and Syntrophobacter genera belonging to the Proteobacteria phylum, Desulfitobacterium in Firmicutes and Caldilinea of Chloroflexi.The SRB of hindgut mainly includes genera of Desulfobulbus,Desulfovibrio,Desulfotalea,Syntrophobacter,Desulfomonile and Sphingomonas of Proteobacteria.The metabolic functional prediction of FAPROTAX database based on high-throughput sequencing results showed that the intestinal SRB of Cyprinus carpio L.was mainly involved in sulfate respiration, respiration of sulfur compounds and chemoheterotrophy.The results of this study provide fundamental basis for further studies on the SRB and their microecological functions in fish intestinal environment.

关 键 词：黄河鲤(Cyprinus carpio L.) 肠道菌群 硫酸盐还原菌(SRB) dsrB高通量 功能预测 

分 类 号：S917.1[农业科学—水产科学]