低氧应激下克氏原螯虾肝胰腺转录组分析  

Transcriptome analysis of the hepatopancreas of Procambarus clarkii under hypoxic stress

作　　者：于瑶瑶 王丹[2] 徐进[2] 徐滨[2] 马宝珊[2] 朱祥云[2] YU Yao-yao;WANG Dan;XU Jin;XU Bin;MA Bao-shan;ZHU Xiang-yun(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Freshwater Biodiversity Conservation,Ministry of Agriculture and Rural Affairs,Wuhan 430223,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院长江水产研究所,农业农村部淡水生物多样性保护重点实验室,武汉430223

出　　处：《淡水渔业》2022年第6期26-35,共10页Freshwater Fisheries

基　　金：蓝色粮仓科技创新项目(2018YFD0901303、2019YFD0900305);中国水产科学研究院基本科研业务费项目(2022XT0103)。

摘　　要：为研究克氏原螯虾(Procambarus clarkii)在低氧应激下机体应对低氧胁迫的分子机制,本研究对低氧应激下克氏原螯虾的肝胰腺进行了转录组测序分析。所得序列经质控、组装后比对到非冗余蛋白库(NR)、蛋白质序列数据库(Swiss-Prot)、蛋白质家族集合数据库(pfam)、蛋白直系同源簇数据库(COG)、基因本体(GO)和生物学通路分析(KEGG)数据库中注释,并进行差异基因聚类分析。结果显示,测序共获得了125496个特异基因(Unigenes),平均长度为760.08 bp。对比正常状态组,显示差异表达基因251个。GO功能富集前十的条目包括组蛋白H3-K36甲基化负调控、黄体溶解等条目。KEGG富集结果显示,低氧应激下差异表达基因富集在钙信号重要通路、磷脂酰肌醇信号通路、缬氨酸、亮氨酸和异亮氨酸的降解,脂肪酸代谢以及糖代谢等通路。同时KEGG通路分析结果显示,环磷酸腺苷(cAMP)、丝裂原活化蛋白激酶(MAPK)和磷脂酰肌醇-3激酶-蛋白激酶B(PI3K-Akt)这三个信号通路在机体适应低氧环境、维持细胞存活中发挥了重要作用。这些结果表明在低氧应激下,克氏原螯虾机体产生各种转录因子调控下游基因表达,进而激活虾体适应低氧应激的一系列生理活动,增强体内能量物质的无氧代谢途径,为机体应对低氧应激提供能量;同时黄体溶解功能富集,提示低氧应激对于性腺发育具有不良影响。Procambarus clarkii has high economic value and is an important characteristic of aquaculture species in China.However, it is susceptible to hypoxia stress and causes significant loss to breeding.The hepatopancreas of P.clarkii under hypoxic stress were analyzed by transcriptome sequencing to study the molecular mechanism of coping with hypoxic stress.In this study, hepatopancreas of P.clarkii under hypoxic stress [DO:(2.5±0.2) mg/L]were analyzed by transcriptome sequencing.After quality control and assembly, the sequences were compared and annotated into NR,Swiss-PROT,PFAM,COG,GO and KEGG databases and cluster analysis of differential genes was performed.The results showed that a total of 125496 Unigenes were sequenced, with an average length of 760.08 bp.Compared with the regular group, 251 differentially expressed genes were found, and the X2 gene of K subfamily K1 was up-regulated significantly.The top 10 items of GO enrichment include negative regulation of histone H3-K36 methylation and luteolysis.KEGG enrichment results showed that differentially expressed genes were enriched in calcium signaling pathway,phosphatidylinositol signaling pathway, valine leucine and isoleucine degradation, fatty acid metabolism and glucose metabolism under hypoxia stress.Meanwhile, KEGG pathway analysis results also showed that the three signaling pathways, cyclic adenosine phosphate, mitogen-activated protein kinase and phosphatidylinositol-3 kinase-protein kinase B,play an important role in the body′s adaptation to hypoxia environment and maintenance of cell survival during hypoxia stress.These results indicated that the body of P.clarkii produced a variety of transcription factors to regulate the expression of downstream genes under hypoxic stress, and then activates a series of physiological activities to adapt to hypoxic stress, enhancing the anaerobic metabolism pathway of energy substances, and providing energy for the body to cope with hypoxic stress.The enrichment of luteolysis was also found, suggesting that hypox

关 键 词：克氏原螯虾(Procambarus clarkii) 低氧应激 转录组测序 能量代谢 信号通路 

分 类 号：S917.4[农业科学—水产科学]