基于飞秒激光超快光谱的生物表界面力学参数全光测量(特邀)  

All-optically Measuring Mechanical Parameters of Bio-surface/interface with Femtosecond Laser Spectroscopy(Invited)

作　　者：张何 许文雄 李奇维 夏传晟 王潇璇 丁海波 徐春祥[1] 崔乾楠 ZHANG He;XU Wenxiong;LI Qiwei;XIA Chuansheng;WANG Xiaoxuan;DING Haibo;XU Chunxiang;CUI Qiannan(State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China)

机构地区：[1]东南大学生物科学与医学工程学院生物电子学国家重点实验室,南京210096

出　　处：《光子学报》2022年第10期208-216,共9页Acta Photonica Sinica

基　　金：国家重点研发计划(No.2017YFA0700503);国家自然科学基金(Nos.61821002,11734005,62075041)。

摘　　要：通过飞秒激光泵浦探测,在多层二硫化钼与生物水凝胶复合界面上,实现了GHz超高频声波的全光产生与时间分辨探测。进一步,采用频谱分析与理论解析手段,获取了生物水凝胶的声速和杨氏模量等力学参数。研究结果为生物表界面力学参数提供了一种全光无损测量方法,可为基于二维半导体的新型光声换能器构建、生物表界面力学参数的成像和超高时空分辨探测技术发展提供理论和实验参考。The mechanical properties and parameters of bio-surface/interface are very important in fundamental researches and applications,such as constructing organ-on-a-chip in order to realize vitro culture of human cells and tissues.To acquire mechanical parameters of bio-surface/interface for real human cells,Atomic Force Microscopy(AFM)is usually employed.The young's modulus of human cells'surface/interface can be obtained by measuring the stress and strain of human cells induced by AFM tip.Obviously,this conventional method is invasive,which might not only cause damage of bio-surface/interface such as cell membrane,but also accompany a low speed for sensing and imaging applications.Besides,ultrasound elastography imaging has been developed to obtain three-dimensional distribution of mechanical parameters for bio-tissues.Unfortunately,the MHz ultrasound waves emitted by conventional ultrasound transducer limit its spatial resolution to micrometer.Hence,developing an accurate,in-situ,noninvasive and quantitative measuring method with high spatiotemporal resolutions to evaluate the mechanical performances has been highly desired.In recent years,layered Two-dimensional(2D)semiconductors,such as Transition Metal Dichalcogenides(TMDs)have presented extraordinary fundamental physical properties as well as good biocompatibility.Van der Waals bondings facilitate their facile integrations with other materials,including bio-materials,to form hetero-interfaces.Most importantly,previous studies have shown that GHz Coherent Acoustic Phonon(CAP)oscillations can be generated under femtosecond laser excitations.Although TMDs show great potential as novel optoacoustic transducers,the effective generation of CAP pulse and experimental measurements of mechanical parameters of bio-surface/interface still need further investigations.In this paper,employing layered 2D semiconductors as GHz optoacoustic transducer,we report a new all-optical technique to noninvasively,accurately,and swiftly measure mechanical parameters of biosurface/interf

关 键 词：飞秒激光 生物表界面 超快光谱 相干声学声子 声速 

分 类 号：O433.1[机械工程—光学工程]