内生真菌对青贮玉米幼苗抗旱性的影响  

Effects of endophytic fungi on drought stresstolerance of silage maize seedlings

作　　者：王英娜 王佳玮 夏依婷 刘涛 李联欢 刘建利 康鹏 李素芬 WANG Yingna;WANG Jiawei;XIA Yiting;LIU Tao;LI Lianhuan;LIU Jianli;KANG Peng;LI Sufen(College of Biological Science and Engineering,North Minzu University,Key Laboratory of Ecological Protection of Agro-pastoral Ecotones in the Yellow River Basin,National Ethnic Affairs Commission of the People’s Republic of China,Ningxia Key Laboratory of Microbial Resources Development and Applications in Special Environment,Yinchuan,Ningxia 750021,China;West Ordos National Nature Reserve Administration,Ordos,Inner Mongolia 014300,China)

机构地区：[1]北方民族大学生物科学与工程学院,国家民委黄河流域农牧交错区生态保护重点实验室,宁夏特殊生境微生物资源开发与利用重点实验室,宁夏银川750021 [2]西鄂尔多斯国家级自然保护区管理局,内蒙古鄂尔多斯014300

出　　处：《干旱地区农业研究》2022年第6期45-55,71,共12页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金项目(31960346);北方民族大学研究生创新项目(YCX21019)。

摘　　要：为了研究干旱胁迫下内生真菌对青贮玉米幼苗生长的影响并筛选可以提高青贮玉米幼苗抗旱性的菌株,开展盆栽试验,将分离自西鄂尔多斯荒漠强旱生孑遗植物四合木、沙冬青、绵刺等植物根系的8株内生真菌和1株内生真菌模式种印度梨形孢,以灌根的方式分别接种到青贮玉米幼苗根系,镜检侵染率后,采用PEG6000模拟重度干旱胁迫培养2周,测定青贮玉米幼苗生理生化指标并计算抗旱系数,利用主成分分析法综合评价内生真菌对青贮玉米幼苗抗旱性的影响。结果表明:所有菌株均能侵入青贮玉米根系形成共生,但侵染率存在差异;在未接菌(E-)组的非胁迫和胁迫植株之间,地上部分鲜重、叶相对含水量、叶绿素含量、根体积、根表面积、根鲜重、根干重、根干物质含量、Pro含量、MDA含量、CAT活性、SOD活性、POD活性等13个指标存在显著差异,基于13个生理指标的主成分分析表明,只有Am04、Tm36、Tm02、印度梨形孢等4株内生真菌可提高青贮玉米幼苗抗旱性,并以菌株Am04最优;而Pm53、Tm30、Pm38、Pm06、Pm57等5株内生真菌并未提高青贮玉米幼苗抗旱性;且侵染率和大多数抗旱性生理指标之间无相关性。因此,不同内生真菌对青贮玉米幼苗抗旱性具有不同程度影响,其中菌株Am04较其他菌株更有利于提高青贮玉米幼苗抗旱能力。To study the effects of endophytic fungi on growth of silage maize seedlings and obtain excellent strain enhancing the drought tolerance of silage maize seedlings,8 strains of endophytic fungi isolated from the roots of three strong xerophytes(Tetraena mongolica、Ammopiptanthus mongolicus、Potaninia mongolica)planting in West Ordos and Piriformospora indica of model species of endophytic fungi were selected as test materials to inoculate into the roots of silage maize seedlings.After two weeks under severe drought stress simulated with PEG6000,the physiological and biochemical indexes of the seedlings were measured and the drought resistance coefficient was calculated.Then the effect of inoculating endophytic fungi on drought resistance of silage maize seedlings was evaluated using principal component analysis method.The results showed that all strains invaded the roots of silage maize seedlings to form symbiosis,but the infection rates were very different between different endophytic fungi strains.There were significant differences in 13 physiological indexes between non-drought stressed and drought stressed group in the uninoculated plants(E-),such as aboveground fresh weight,leaf relative water content,chlorophyll content,root volume,root surface area,root fresh weight,root dry weight,root dry matter content,Pro content,MDA content,CAT activity,SOD activity and POD activity.The principal component analysis based on 13 physiological indexes demonstrated that only 4 endophytic fungi(Am04,Tm36,Tm02 and Piriformospora indica)improved the drought resistance of silage maize seedlings.The strains Am04 was the best.However,the other 5 endophytic fungi(Pm53,Tm30,Pm38,Pm06 and Pm57)did not improve the drought resistance of silage maize seedlings.There was no correlation between infection rate and most physiological indexes of drought resistance.Therefore,different endophytic fungi strains had different effects on the drought resistance of silage maize seedlings.The strain Am04 improved the drought resistance of silage

关 键 词：内生真菌 强旱生植物 青贮玉米 幼苗 抗旱性 

分 类 号：Q939.96[生物学—微生物学]