9株野生根瘤菌的分离鉴定及^(60)Co耐受·吸附性研究  

Study on Isolation and Identification of 9 Wild Rhizobium Strains and ^(60)Co Tolerance and Adsorption

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作  者:伏毅[1] 刘绵学[1] 王艳[1] 高鹏[1] 黄敏[1] FU Yi;LIU Mian-xue;WANG Yan(Sichuan Institute of Atomic Energy,Sichuan Province Key Laboratory of Irradiation Preservation,Chengdu,Sichuan 610101)

机构地区:[1]四川省原子能研究院,辐照保藏四川省重点实验室,四川成都610101

出  处:《安徽农业科学》2022年第21期1-5,共5页Journal of Anhui Agricultural Sciences

基  金:四川省科技计划项目(2016SZ0075,2017JY0242,2021JDR0014)。

摘  要:从野生豆科植物根瘤中分离、纯化、筛选出生物活性好的根瘤菌进行种属鉴定,模拟检测其对^(60)Co的耐受性和吸附能力,为放射性钴污染水体、土壤生物修复提供目标菌株。用平板培养法从不同植物根瘤中分离到根瘤菌株9株,使用多位点序列(16S-IGS、nifH、recA)分析方法对获得的根瘤菌进行分子鉴定,并检测理化性质,在不同浓度^(59)Co培养基中培养根瘤菌菌株,检测其对^(59)Co的耐受性和吸附能力,并使用^(60)Co辐射源装置对根瘤菌株进行照射处理,检测其对放射性的耐受能力。结果表明,9株菌株均为革兰氏阴性菌,其中1#、2#、6#、7#菌株为共生固氮菌、慢生菌,3#、4#、5#、8#、9#为联合固氮菌、快生菌;4#、5#、7#、8#、9#菌株具有氨苄抗性。经分子鉴定,1#、2#、6#为Bradyrhizobium japonicum,3#、8#为Sinorhizobium meliloti,4#、5#为Rhizobium sp.,7#为Bradyrhizobium yuanmingense,9#为Rhizobium huautlense;5#菌株对^(59)Co的耐受能力最好,7#最差。初始^(59)Co浓度为1 g/L和吸附时间1.5 h条件下,9#菌株对培养基中^(59)Co的残余量最低,说明该菌株的清除能力最强,达到15%。^(60)Co辐射源装置对9个根瘤菌株进行照射处理,所有菌株均不能耐受1~10 kGyγ射线辐照剂量处理。从试验结果来看,5#菌株和9#菌株对^(59)Co分别表现出较好的耐受性和吸附性,在修复低放射性^(60)Co污染水体、土壤中具有一定的潜力。Rhizobia with good biological activity were isolated,purified and identified from the root nodules of wild legumes,and their tolerance and adsorption capacity to ^(60)Co were simulated to provide target strains for the bioremediation of water and soil polluted by radioactive cobalt.9 Rhizobium strains were isolated from different plant root nodules by plate culture method.The Rhizobium strains were identified by multi site sequence(16S-IGS,nifH,recA)analysis,and their physical and chemical properties were detected.The tolerance and adsorption of the Rhizobium strains were detected in ^(59)Co medium with different concentrations.The radioactivity tolerance of the Rhizobium strains were detected with ^(60)Co radiation source device.The results showed 9 strains were Gram-negative bacteria,1#,2#,6#,7#strains were symbiotic nitrogen fixing bacteria,slow-growing bacteria,3#,4#,5#,8#,9#strains were combined nitrogen fixing bacteria and fast-growing bacteria;4#,5#,7#,8#,9#strains had ampicillin resistance.Through molecular biology identification,1#,2#,6#were Bradyrhizobium japonicum,3#,8#were Sinorhizobium meliloti,4#,5#were Rhizobium sp.,7#was Bradyrhizobium yuanmingense and 9#was Rhizobium huautlense.5#strain had the best tolerance to ^(59)Co and 7#the worst.Under the condition that the initial ^(59)Co concentration was 1 g/L and the adsorption time was 1.5 h,the 9#strain had the lowest residual amount of ^(59)Co in the medium,indicating that the strain had the strongest scavenging ability,reaching 15%.9 strains were irradiated by ^(60)Co radiation source device,and all strains were not survive by 1-10 kGyγradiation dose treatment.The results indicated that the 5#strain and the 9#strain showed good tolerance and adsorption to ^(59)Co,respectively,and had a certain potential in the restoration of low-radioactive ^(60)Co polluted water and soil.

关 键 词:^(60)Co 根瘤菌 生物修复 分离鉴定 耐受性 吸附性 

分 类 号:X591[环境科学与工程—环境工程]

 

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