外源植物激素对胶球藻生物量和油脂富集的影响  

Effects of exogenous phytohormones on biomass and lipid accumulation in Coccomyxa subellipsoidea

作　　者：李玉芹[1] 齐振华 郝丽阳 樊怡雯 LI Yuqin;QI Zhenhua;HAO Liyang;FAN Yiwen(Department of Biological and Food Engineering,College of Chemical Engineering,Xiangtan University,Xiangtan 411105,China)

机构地区：[1]湘潭大学化工学院生物与食品工程系,湖南湘潭411105

出　　处：《湘潭大学学报（自然科学版）》2022年第5期10-20,共11页Journal of Xiangtan University(Natural Science Edition)

基　　金：湖南省自然科学基金(2021JJ30657);湖南省教育厅项目(19C1775,20A472);国家级大学生创新创业训练计划项目(S20211053020)。

摘　　要：该文探讨了胶球藻在不同生长时期外源添加水杨酸(SA)、茉莉酸甲酯(MeJA)和脱落酸(ABA)对其生物量和油脂富集的影响.研究显示,胶球藻在生长初期添加4 mg/L ABA诱导培养168 h获得最佳生物量、油脂含量和油脂产量分别为6.77 g/L、70.25%和4.76 g/L,分别是生长初期添加10 mg/L SA培养168 h最佳生物量、油脂含量和油脂产量的1.57、1.19和1.88倍,也比稳定期添加5 mg/L MeJA诱导48 h的最大生物量、油脂含量和油脂产量分别高1.15、1.16和1.34倍.ABA诱导胶球藻以C16和C18的脂肪酸为主,其中α-亚麻酸和亚油酸分别占总脂肪酸的30.06%和37.18%,较对照组分别提高了22.8%和17.66%.进一步研究显示,ABA显著下调胶球藻固碳路径PGK活性,同时上调甘油磷脂通路GPD和GDPD、丙酮酸路径ME、油脂合成积累路径FAS,以及TAG合成路径MGAT活性,使得光合固碳路径减弱,中心碳代谢流流向油脂路径,加之甘油酯路径增强,为油脂合成提供了能量和前驱物,从而协同促进了胶球藻油脂富集.研究结果为揭示激素诱导微藻油脂积累机制及拓展食源性胶球藻脂质应用奠定基础.The influences of exogenous salicylic acid(SA),methyl jasmonate(MeJA),and abscisic acid(ABA)on biomass and lipid content of Coccomyxa subellipsoidea(C.subellipsoidea)at different growth stages were investigated.The results showed that the biomass,lipid content,and lipid yield of C.subellipsoidea in early growth stage supplemented with 4 mg/L ABA for 168 h achieved the highest values by 6.77 g/L,70.25%,and 4.76 g/L.These values were 1.57-,1.19-,1.88-fold higher than that of C.subellipsoidea in early growth stage supplemented with 10 mg/L SA for 168 h as well as 1.15-,1.16-,and 1.34-fold more than that of C.subellipsoidea in stable growth stage supplemented with 5 mg/L MeJA for 48 h.The fatty acids in C.subellipsoidea cells in early growth stage supplemented with 4 mg/L ABA for 48 h were mainly composed of C16 and C18.α-linolenic acid and linoleic acid accounted for 30.06%and 37.18%of the total fatty acids,representing 22.8%and 17.66%more than that of control.Furtherly,exogenous ABA significantly down-regulated PGK in Calvin cycle,concurrently up-regulated GPD and GDPD in glycerolipid pathway,pyruvate pathway ME,fatty acid pathway FAS,and MGAT in TAG synthesis pathway.These weakened photosynthetic carbon sequestration to drive carbon flow into lipid pathway as well as enhanced glyceride pathway to provide energy and precursors for lipid accumulation in C.subellipsoidea.The outcomes of this work provided a basis for revealing lipid accumulation induced by hormones in microalgae and expanding applications of foodborne C.subellipsoidea lipids.

关 键 词：胶球藻 脱落酸 生物量 油脂富集 脂肪酸 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ92[轻工技术与工程—食品科学与工程]