产后抑郁患者肠道微生物的特征  

Intestinal microbiome changes in patients with postpartum depression

作　　者：杨再 张玉丹 穆鑫 裴美丽[4] 高媛 范雅娟 赵斌斌 马现仓[1] YANG Zai;ZHANG Yudan;MU Xin;PEI Meili;GAO Yuan;FAN Yajuan;ZHAO Binbin;MA Xiancang(Department of Psychiatry,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061;Department of Translational Medicine,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061;Reproductive Center,Northwest Women and Children’s Hospital,The Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061;Department of Gynecology&Obstetrics,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学第一附属医院精神心理卫生科,陕西西安710061 [2]西安交通大学第一附属医院转化医学中心,陕西西安710061 [3]西安交通大学附属西北妇女儿童医院生殖中心,陕西西安710061 [4]西安交通大学第一附属医院妇产科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2022年第6期879-884,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金面上项目(No.81771471)。

摘　　要：目的分析产后抑郁(postpartum depression,PPD)患者肠道微生物的变化,探讨PPD与肠道微生物之间的关系。方法共招募了60例产后受试者参加本研究,使用爱丁堡产后抑郁量表(EPDS)对产妇的抑郁状态进行评分,得分≥13的入组为产后抑郁组(PPD组),得分<13的入组为产后健康对照组(PPHC组)。分别收集这60例受试者的粪便,提取粪便的全基因组DNA进行16S rDNA测序,并获得组间菌群多样性变化数据,分析肠道微生物的改变与PPD的潜在相关性。结果PPD患者的微生物明显减少(P<0.001),并且α多样性的Chao1指数(P<0.001)和ACE指数(P<0.001)显著降低。两组间β多样性也存在显著差异。组间差异菌分析发现,Acetanaerobacterium、Adlercreutzia、Allobaculum、Alloprevotella、Bifidobacterium、Christensenella、Escherichia、Eubacterium、Faecalicatena、Fusobacterium、Haemophilus、Intestinimonas、Lactobacillus、Megamonas、Monoglobus、Muribaculum、Oscillospira、Paraprevotella、Streptococcus、Raoultibacter、Ruminococcus、Stomatobaculum等菌在PPHC组显著富集,而Kineothrix,Lachnoclostridium,Acinetobacter,Aquisphaera,Enterococcus,Mucispirillum在PPD组中显著富集。RDA/CCA分析发现,PPD EPDS得分与Prevotella,Kineothrix,Alistipes呈正相关,而与Lachnospira呈负相关。结论PPD患者的肠道菌群发生明显紊乱,且与PPD症状评分之间存在相关性。因此,肠道微生物标志物可能有助于PPD患者的诊断和治疗。Objective To analyze the changes of gut microbes in patients with postpartum depression so as to explore the relationship between postpartum depression and gut microbes.Methods A total of 60 postpartum s ubjects were recruited to participate in this study.The depression status of the participants was scored using the Edinburgh Postpartum Depression Scale(EPDS).Those with a score≥13 were included in the postpartum depression group(PPD group),while those with a score less than 13 were included in the postpartum healthy control group(PPHC group).The feces of these 60 subjects were collected,and the fecal whole genome DNA was extracted for 16S rDNA sequencing.The data of changes in the bacterial diversity between the groups were obtained,and the possible correlation between the changes of intestinal microbes and postpartum depression was analyzed.Results T he number of microorganisms in PPD patients was significantly reduced(P<0.001);the Chao1 index(P<0.001)and ACE index(P<0.001)ofαdiversity decreased significantly.There were also significant differences inβdiversity between the two groups.Analysis of the bacteria in the groups showed that Acetanaerobacterium,Adlercreutzia,Allobaculum,Alloprevotella,Bifidobacterium,Christensenella,Escherichia,Eubacterium,Faecalicatena,Fusobacterium,Haemophilus,Intestinimonas,Lactobacillus,Megamonas,Monoglobumus,Muribaculum,Oscillospira,Paraprevotella,Streptococcus,Raoultibacter,Ruminococcus and Stomatobaculum were significantly enriched in PPHC group.In contrast,Kineothrix,Lachnoclostridium,Acinetobacter,Aquisphaera,Enterococcus,and Mucispirillum were enriched in PPD group.RDA/CCA analysis showed that EPDS was positively correlated with Prevotella,Kineothrix,and Alistipes,but negatively correlated with Lachnospira.Conclusion This study found that the intestinal flora of patients with postpartum depression was significantly d isrupted,and there was a correlation between the intestinal flora and postpartum depression symptom score.Therefore,intestinal microbial markers may contribu

关 键 词：产后抑郁 肠道微生物 16S测序 爱丁堡产后抑郁量表(EPDS) 益生菌 

分 类 号：R714.6[医药卫生—妇产科学] R749.4[医药卫生—临床医学]