A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence antiquenching  

作　　者：Lingyuan Xu Xiuyuan Zhang A.M.Abd El-Aty Yuanshang Wang Zhen Cao Huiyan Jia J.-Pablo Salvador Ahmet Hacimuftuoglu Xueyan Cui Yudan Zhang Kun Wang Yongxin She Fen Jin Lufei Zheng Baima Pujia Jing Wang Maojun Jin Bruce D.Hammock 

机构地区：[1]Institute of Quality Standard and Testing Technology for Agro-Products,Chinese Academy of Agricultural Sciences,Beijing,100081,China [2]Department of Pharmacology,Faculty of Veterinary Medicine,Cairo University,Giza,12211,Egypt [3]Department of Medical Pharmacology,Medical Faculty,Ataturk University,Erzurum,25240,Türkiye [4]Institute of Livestock and Poultry,Ningbo Academy of Agricultural Sciences,Ningbo,Zhejiang,315040,China [5]Nanobiotechnology for Diagnostics Group,Instituto de Química Avanzada de Cataluna,IQAC-CSIC,Barcelona,08034,Spain [6]CIBER de Bioingeniería,Biomateriales y Nanomedicina(CIBER-BBN),Madrid,28029,Spain [7]Inspection and Testing Center of Agricultural and Livestock Products of Tibet,Department of Agriculture and Rural Affairs of Tibet Autonomous Region,Lhasa,850000,China [8]Department of Entomology&Nematology and the UC Davis Comprehensive Cancer Center,University of California,Davis,CA,95616,USA

出　　处：《Journal of Pharmaceutical Analysis》2022年第4期637-644,共8页药物分析学报（英文版）

基　　金：supported by the Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural Sciences(Grant No.:Y2021PT05);National Institute of Environmental Health Science Superfund Research Program(Grant No.:P42 ES004699);National Academy of Sciences(Subaward No.:2000009144);Ningbo Innovation Project for Agro-Products Quality and Safety(Grant No.:2019CXGC007).

摘　　要：Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and rice.Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs.DNA oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal amplification.The detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the fluorophore.The resulting fluorescence signal enables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concentration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,respectively.The mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%-17.6%,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

关 键 词：Organophosphate pesticides Fluorescence anti-quenching Gold nanoparticles DNA-RNA hybridization DNA oligonucleotides Ribonuclease H 

分 类 号：R446.1[医药卫生—诊断学]