绿豆种质资源枯萎病抗性鉴定及抗性资源筛选  

Identification and Screening for Resistance Germplasm Resources to Fusarium Wilt in Mungbean

作　　者：沈颖超 张志肖[1] 孙素丽[2] 王彦[1] 范保杰[1] 刘长友[1] 王珅 苏秋竹[1] 时会影 朱振东[2] 田静[1] SHEN Ying-chao;ZHANG Zhi-xiao;SUN Su-li;WANG Yan;FAN Bao-jie;LIU Chang-you;WANG Shen;SU Qiu-Zhu;SHI Hui-ying;ZHU Zhen-dong;TIAN Jing(Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Scieces/Hebei Laboratory of Crop Genetic and Breeding,Shijiazhuang 050035;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,石家庄050035 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《植物遗传资源学报》2022年第6期1660-1669,共10页Journal of Plant Genetic Resources

基　　金：国家重点研发专项(2019YFD1000703);国家食用豆产业技术体系(CARS-08-G03);河北省杂粮杂豆种业科技创新团队(21326305D);河北省现代产业技术体系杂粮杂豆创新团队(HBCT2018070203)。

摘　　要：绿豆枯萎病是影响绿豆产量最严重的病害之一。筛选苗期抗性资源,培育抗病品种对枯萎病防治具有重要意义。本研究采用剪根浸根接种法,对来自全国18个省市及国外的215份绿豆核心种质资源和85份绿豆新品系进行了苗期枯萎病抗性鉴定。结果显示,不同地区种质间的枯萎病抗性水平存在差异。国内产区中,东北、华东、华中地区约50%的种质具有枯萎病抗性;华北地区抗枯萎病种质占比40.4%;西北、西南和华南地区种质抗病水平较高;国外材料抗病种质占比40.0%。本研究共筛选出17份高抗(HR)枯萎病种质资源,并利用部分材料建立了枯萎病抗性研究RIL群体;6份高抗高代品系材料,在田间全生育期表现高抗且农艺性状优异。本研究期望为今后抗枯萎病绿豆新品种选育及抗性遗传相关研究提供优异资源和理论依据。Fusarium wilt is one of the most serious diseases causing the yield loss in mungbean.Identification of resistant resources at the seedling stage and breeding for resistant mungbean varieties become of importance.In this study,215 mungbean core germplasm resources and 85 new breeding lines were tested for resistance against fusarium wilt at the seedling stage.The differences in resistance to fusarium wilt were observed among germplasm resources collected from different geographical regions.For these germplasm resources that were collected from different regions of China,ca.50%of germplasm resources in Northeast,East and Central China turned to be resistant,while 40.4%of genotypes in North China were resistant.If compared with those of other regions,a higher disease resistance was observed in germplasm collected from Northwest,Southwest and South China.40.0%of the exotic germplasm were found to be resistant.seventeen accessions showing high resistance(HR)to fusarium wilt were found at the seedling stage,and of them several had been used to generate RIL populations.Six breeding lines showing high resistant and excellent agronomic performance in lifecycle were obtained.Collectively,this study provided excellent resources and theoretical basis in breeding of new mungbean varieties resistant to fusarium wilt and future decoding of resistance genes.

关 键 词：绿豆 枯萎病 种质资源 抗性鉴定 

分 类 号：S435.22[农业科学—农业昆虫与害虫防治]