基于单片段代换系的水稻芽期耐冷QTL定位和聚合  

Identification and Pyramiding of QTLs for Cold Tolerance at the Bud Bursting Stage by Use of Single Segment Substitution Lines in Rice(Oryza sativa L.)

作　　者：杨梯丰[1] 董景芳[1] 赵均良[1] 周炼 杨武 马雅美 王健 陈洛 陈建松 吴伟 李雯慧 刘斌[1] 张少红[1] YANG Ti-feng;DONG Jing-fang;ZHAO Jun-liang;ZHOU Lian;YANG Wu;MA Ya-mei;WANG Jian;CHEN Luo;CHEN Jian-song;WU Wei;LI Wen-hui;LIU Bin;ZHANG Shao-hong(Rice Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology for Rice Breeding/Guangdong Rice Engineering Laboratory,Guangzhou 510640)

机构地区：[1]广东省农业科学院水稻研究所/广东省水稻育种新技术重点实验室/广东省水稻工程实验室,广州510640

出　　处：《植物遗传资源学报》2022年第6期1726-1736,共11页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(32072047,31501389);广东省基础与应用基础研究基金(2020A1515011051,2022A1515012135);广州市科技计划项目(201804020078,202102021004);广东省科技计划项目(2017A020208022,2020B1212060047,2020B0202090003)。

摘　　要：芽期耐冷性是华南双季稻地区水稻育种的一个重要目标。虽然水稻芽期耐冷QTL的标记定位已取得了一定的进展,但是这些QTL/基因尚未在水稻育种中得到有效的应用。定位稳定表达的芽期耐冷QTL,开展QTL聚合育种是水稻芽期耐冷性育种取得突破的关键。在本研究中,利用以粳稻IR65598-112-2为供体,籼稻华粳籼74为受体构建的单片段代换系(SSSL)开展芽期耐冷QTL定位,并进行聚合育种。通过评价SSSL与受体华粳籼74的芽期耐冷性差异,定位了2个稳定的芽期耐冷QTLs(qCTBB-3和qCTBB-12)。试验表明,分别携带有耐冷QTL qCTBB-3和qCTBB-12的SSSL在冷处理后都比华粳籼74表现出更高的幼苗成活率。通过代换作图,发现在qCTBB-3区间存在2个紧密连锁的耐冷QTLs(qCTBB-3a和qCTBB-3b)。利用本研究携带qCTBB-3a/qCTBB-3b的单片段代换系和前期研究鉴定出的芽期耐冷QTL qCTBB-6的单片段代换系为亲本进行杂交,通过分子标记辅助选择,获得了2份含有这3个QTL的聚合系。耐冷性评价表明,来源于两个供体/亲本的QTL不存在显著的上位性效应,聚合系的芽期耐冷性较亲本显著增强。可见,通过聚合芽期耐冷QTLs qCTBB-3a/qCTBB-3b和qCTBB-6能显著提高水稻芽期的耐冷性,获得的QTL及三耐冷QTL聚合系为水稻芽期耐冷性分子育种提供了优良的基因资源和亲本材料。Cold tolerance at the bud bursting stage is an important target of rice breeding in doublecropping rice region of South China. The achievements have been made in mapping QTL,whereas these QTL have not been effectively used in rice breeding. Identification and pyramiding of QTL are of interest to achieve a breakthrough in rice breeding for cold tolerance at bud bursting stage. In this study,single segment substitution lines(SSSLs)derived from a cross between cold-tolerant japonica variety IR65598-112-2 and a popular indica variety Huajingxian 74 were used to detect and pyramid QTL for cold tolerance at the bud bursting stage.Two QTL qCTBB-3 and qCTBB-12 were identified by evaluating the difference of cold tolerance between SSSL and their recurrent parent Huajingxian 74. The SSSLs carrying qCTBB-3 or qCTBB-12 showed higher seedling survival percentage than that of their recurrent parent Huajingxian 74 after cold treatment. Through substitution mapping,two linked cold-tolerant QTL(qCTBB-3a and qCTBB-3b)were found in qCTBB-3 region.Furthermore,QTL pyramiding was performed by inter-cross of SSSLs carrying the cold-tolerant QTL(qCTBB-3a/qCTBB-3b,and qCTBB-6 identified in the previous study)and marker-assisted selection(MAS). The lines harboring three QTL showed cumulative effects on cold tolerance. Collectively,by identification of two coldtolerant QTL and generation of the pyramiding lines with three QTL,this study provided the genes and parental lines in molecular breeding for cold tolerance at the bud bursting stage in rice.

关 键 词：芽期耐冷性 QTL定位 分子标记辅助选择 聚合育种 单片段代换系 水稻 

分 类 号：S511[农业科学—作物学]