小麦Lhc基因家族鉴定与表达模式分析  

Identification and Expression Pattern Analysis of Lhc Gene Family Members in Wheat

作　　者：赵奇 茹京娜 李宜统 王超[2] 徐兆师[2] 王睿辉[1] ZHAO Qi;RU Jing-na;LI Yi-tong;WANG Chao;XU Zhao-shi;WANG Rui-hui(College of Agronomy,Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071001;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops,Ministry of Agriculture,Beijing 100081)

机构地区：[1]河北农业大学农学院/华北作物改良与调控国家重点实验室,保定071001 [2]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部麦类生物学与遗传育种重点实验室,北京100081

出　　处：《植物遗传资源学报》2022年第6期1766-1781,共16页Journal of Plant Genetic Resources

基　　金：河北省小麦现代种业科技创新团队建设项目。

摘　　要：捕光叶绿素a/b结合蛋白(Lhc,light-harvesting chlorophyll a/b-binding protein)直接影响光合系统的效率和作物产量。小麦作为我国主要粮食作物之一,生长发育过程中,非生物胁迫严重影响小麦的产量和品质。为了系统研究小麦Lhc基因家族的特性,本研究对普通小麦中的Lhc基因家族成员进行了鉴定和生物信息学分析,并选择其中的差异显著或高表达基因进行了定量表达分析。结果显示,从小麦基因组中共鉴定到96个Lhc基因,除4个基因(TaLhca5.1、TaLhcb1.31、TaLhcb1.39和TaLhcb1.29)未定位到具体染色体外,其余基因分布于除3A和3D外的19条染色体上;这些Lhc基因在系统发育上分属于3个亚家族,处于同一分支的基因具有相似的基因结构与motif;在Lhc基因的启动子区域,发现存在大量的胁迫响应元件和生长发育相关响应元件;Lhc基因在根、茎、叶、穗和籽粒等器官中的表达模式不同,其中在叶中的表达较高;在所选的4个基因中,TaLhca3.3、TaLhcb1.7和TaLhcb1.20的表达量呈现先升高后降低的趋势,TaLhcb1.46呈下调表达趋势。Light harvesting chlorophyll a/b-binding protein(Lhc)directly affects the efficiency of photosynthetic system and crop yield.Wheat is one of the staple food crops in China,while the abiotic stresses seriously destabilize the yield and quality in its lifecycle.In order to systematically study the characteristics of wheat Lhc gene family members,in this study,the bioinformatics analysis and the transcriptional profile analysis of differently expressed genes or highly expressed genes were carried out.96 Lhc genes were identified based on wheat reference sequences.Except that four genes(TaLhca5.1,TaLhcb1.31,TaLhcb1.39 and TaLhcb1.29)were not assigned to the chromosomes,the remaining 92 genes were mapped on 19 chromosomes of wheat except 3A and 3D.These Lhc genes belong to three subfamilies in phylogeny,with each family having the similarity on gene structure and motif.In the promoter region of Lhc genes,there existed a large number of stress response elements and growth and development related response elements.Expression patterns of Lhc gene are different in root,stem,leaf,spike and grain,of which higher expression was observed in leaves.Transcripts of genes TaLhca3.3,TaLhcb1.7 and TaLhcb1.20 increased initially,followed by a decrease,whereas a decrease on gene expression of the gene TaLhcb1.46 was detected.

关 键 词：小麦 Lhc基因家族 表达分析 非生物胁迫 

分 类 号：S512.1[农业科学—作物学]