杞月茶对卵巢储备功能下降大鼠卵巢组织Bax和Bcl-2蛋白表达的影响  

Effects of Qiyue Tea on the expression of Bax and Bcl 2 proteins in ovarian tissue of rats with decreased ovarian reserve

作　　者：肖慧 刘宇[1] 朱英英 李军[1] Xiao Hui;Liu Yu;Zhu Yingying;Li Jun(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院妇科/郭志强传承工作室/北京中医药薪火传承“3+3工程”郭志强名医传承工作站,100700

出　　处：《疑难病杂志》2022年第11期1180-1185,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：2018国家重点研发计划课题任务(2018YFC1704106);北京中医药大学新奥奖励基金课题(2019-XAJLJJ-013);北京中医药大学东直门医院三名工程教学类资助基金青年教学名师项目(405120413)。

摘　　要：目的探讨杞月茶治疗卵巢储备功能下降大鼠的潜在机制。方法2021年3—5月于北京中医药大学东直门医院中心实验室进行实验。6周龄SD大鼠24只,随机数字表法分为空白组、模型组、杞月茶组、戊酸雌二醇片(补佳乐)组,每组6只。除空白组外,其余大鼠腹腔注射VCD 160 mg/kg制备卵巢储备功能下降(DOR)模型,造模成功后,杞月茶组予杞月茶24 g/kg剂量灌胃,补佳乐组予补佳乐0.12 mg/kg灌胃,空白组及模型组予生理盐水3 ml/kg灌胃,连续灌胃4周。干预结束后,ELISA检测各组大鼠血清促卵泡激素(FSH)、雌二醇(E_(2))、抗苗勒管激素(AMH)的含量,取卵巢组织采用HE染色观察卵巢形态,免疫组织化学法检测卵巢Bax与Bcl-2蛋白表达,蛋白印迹法检测卵巢Bcl-2/Bax表达水平。结果与空白组比较,模型组大鼠中初次级卵泡减少,闭锁卵泡增多;杞月茶组及补佳乐组初次级卵泡及成熟卵泡较多,闭锁卵泡较模型组减少。与空白组比较,模型组卵巢体质量指数、AMH水平降低,FSH水平升高(P<0.05);与模型组比较,杞月茶组及补佳乐组卵巢体质量指数、AMH水平升高,FSH水平降低(P<0.05)。Bax蛋白主要表达在颗粒层细胞、黄体及间质,模型组大鼠较空白组表达升高,杞月茶组及补佳乐组较模型组表达降低(P<0.05)。模型组Bcl-2/Bax比值较空白组降低(P<0.05);杞月茶组及补佳乐组Bcl-2/Bax比值高于模型组(P<0.05)。结论杞月茶可降低DOR大鼠血清中FSH水平、升高AMH水平,与下调卵巢组织中细胞凋亡因子Bax的表达有关。Objective To explore the potential mechanism of Qiyue Tea in the treatment of rats with decreased ovarian reserve.Methods From March to May 2021,experiments will be conducted in the central laboratory of Dongzhimen Hospital,Beijing University of Traditional Chinese Medicine.Twenty-four 6-week-old SD rats were randomly divided into blank group,model group,Qiyue Tea group,and estradiol valerate tablet(Progalo)group,with 6 rats in each group.Except for the blank group,the other rats were intraperitoneally injected with VCD 160 mg/kg to establish a model of decreased ovarian reserve(DOR).The rats in the control group were given 0.12 mg/kg of Procyone by gavage,and the blank group and the model group were given 3 ml/kg of normal saline by gavage for 4 weeks.After the intervention,the serum levels of follicle-stimulating hormone(FSH),estradiol(E_(2))and anti-Müllerian hormone(AMH)were detected by ELISA.The protein expressions of Bax and Bcl 2 in the ovary were detected,and the expression level of Bcl 2/Bax in the ovary was detected by Western blotting.Results Compared with the blank group,the number of primary and secondary follicles in the model group decreased and the number of atretic follicles increased;the Qiyue Tea group and the Bujiale group had more primary and mature follicles,and fewer atretic follicles than the model group.Compared with the blank group,the ovarian BMI and AMH levels in the model group decreased,while the FSH level increased(P<0.05).FSH level decreased(P<0.05).Bax protein was mainly expressed in the granulosa cells,corpus luteum and interstitium.Compared with the blank group,the expression of Bax protein in the model group was higher than that in the blank group.The ratio of Bcl 2/Bax in the model group was lower than that in the control group(P<0.05);the ratio of Bcl 2/Bax in the Qiyue Tea group and Bujiale group was higher than that in the model group(P<0.05).Conclusion Qiyue Tea can reduce the level of FSH and increase the level of AMH in serum of DOR rats,which is related to down-regulat

关 键 词：卵巢储备功能下降 杞月茶 性激素 BCL-2蛋白 BAX蛋白 大鼠 

分 类 号：R711.75[医药卫生—妇产科学]