新生儿B族链球菌定植菌株与侵袭性感染菌株血清型、α样蛋白基因及菌毛岛基因特征分析  

Serotype,α-like protein gene and pilus islands gene characteristics of colonization and invasive infection strains of neonatal group B streptococci

作　　者：刘建瑜 陈继昌 傅锦坚 LIU Jianyu;CHEN Jichang;FU Jinjian(Department of Clinical Laboratory,Guiping People′s Hospital,Guigang,Guangxi 537200,China;Department of Prevention and Health,Liuzhou Maternal and Child Health Hospital,Liuzhou,Guangxi 545001,China)

机构地区：[1]桂平市人民医院检验科,广西贵港537200 [2]柳州市妇幼保健院预防保健科,广西柳州545001

出　　处：《检验医学与临床》2022年第22期3041-3045,共5页Laboratory Medicine and Clinic

基　　金：广西壮族自治区自然科学基金项目(2019GXNSFAA185021);广西壮族自治区柳州市科技局科技攻关与新产品试制项目(2019BJ10601)。

摘　　要：目的探讨新生儿B族链球菌(GBS)定植菌株和侵袭性感染菌株的分子特征。方法对2017年1月至2020年6月检出的新生儿GBS菌株进行抗菌药物敏感性试验、耐药基因、α样蛋白基因、菌毛岛基因和血清型检测。结果共检出44例GBS菌株,其中感染组12例(27.3%),定植组32例(72.7%)。感染组早产儿比例高于定植组(P=0.004)。GBS菌株的多重耐药率为88.6%。感染组携带菌毛岛基因PI2b和α样蛋白基因rib的比例高于定植组(P<0.05);定植组携带菌毛岛基因PI2a和α样蛋白基因alphaC的比例高于感染组(P<0.05)。定植组血清型Ⅰa型比例高于感染组(P<0.05),而感染组血清型Ⅲ型比例高于定植组(P<0.05)。四环素耐药基因tetT更常见于血清型Ⅰa型菌株(P=0.043);alphaC基因常见于血清型Ⅰa型菌株(P<0.001);rib基因常见于血清型Ⅲ型菌株(P<0.001)。对应分析结果显示,α样蛋白基因rib、alp2/3常见于血清型Ⅲ型菌株,而α样蛋白基因alphaC更常见于血清型Ⅰa型菌株(P<0.001);血清型Ⅲ型菌株更容易携带菌毛岛基因PI2b,而血清型Ⅰa型菌株更容易携带菌毛岛基因PI2a(P=0.004)。结论新生儿GBS菌株的多重耐药率较高,GBS菌株的α样蛋白基因和菌毛岛基因在不同的血清型菌株中分布存在差异性。Objective To explore the molecular characteristics of group B streptococci(GBS)isolated from neonatal colonization and invasive infection strains.Methods Antibacterial drug sensitivity test,drug resistance gene,α-like protein gene,pilus islands gene and serotype test were carried out for GBS strains of newborns from January 2017 to June 2020.Results A total of 44 cases of GBS were detected,of which 12 cases(27.3%)were in the infection group,32 cases were in the colonization group(72.7%).The proportion of premature infants in the infection group was higher than that in the colonization group(P=0.004).The multiple drug resistance rate of GBS strains was 88.6%.The proportion of pilus islands gene PI2b andαlike protein gene rib in the infection group was higher than that in colonization group(P<0.05).The proportion of pilus islands gene PI2a andαlike protein gene alphaC in the colonization group was higher than that in the infection group(P<0.05).The proportion of serotypeⅠa in the colonization group was higher than that in the infection group,while the proportion of serotypeⅢin the infection group was higher than that in the colonization group(P<0.05).Tetracycline resistance gene tetT was more common in serotypeⅠa strains(P=0.043),alphaC gene was common in serotypeⅠa strains(P<0.001),and rib gene was common in serotypeⅢstrains(P<0.001).The corresponding analysis results show that,αlike protein gene rib was more common in serotypeⅢstrains,whileαlike protein gene alphaC was more common in serotypeⅠa strains(P<0.001).SerotypeⅢstrains were more likely to carry pilus islands gene PI2b,while serotypeⅠa strains were more likely to carry pilus islands gene PI2a(P=0.004).Conclusion The multiple drug resistance rate of neonatal GBS strains is high,and the distribution ofα-like protein gene and pilus islands gene in GBS strains with different serotypes is different.

关 键 词：B族链球菌 新生儿 耐药基因 血清型 抗菌药物耐药性 

分 类 号：R446.5[医药卫生—诊断学]