GATA3、IL-13基因多态性和IL-13水平与小儿结核病易感性的关系研究  

Relationship between GATA3,IL-13 gene polymorphism and IL-13 level and susceptibility to tuberculosis in children

作　　者：杨英阁[1] YANG Yingge(The First Affiliated Hospital of Nanyang Medical College of Henan Province,Nanyang,Henan 473052,China)

机构地区：[1]河南省南阳医学高等专科学校第一附属医院,河南南阳473052

出　　处：《检验医学与临床》2022年第22期3098-3102,共5页Laboratory Medicine and Clinic

摘　　要：目的探讨GATA3、白细胞介素(IL)-13基因多态性和IL-13水平与小儿结核病易感性的关系。方法随机选取2019年12月至2020年12月在该院诊治的414例小儿结核病患儿作为观察组,另选取456例同期来该院诊治的无肺结核感染的上呼吸道感染患儿作为对照组。提取静脉血DNA,采用Taq-man荧光定量聚合酶链反应技术对GATA3基因的rs422628位点和IL-13基因的rs20541位点进行检测,采用酶联免疫吸附试验检测IL-13水平。统计不同基因型分布频率,并分析不同单核苷酸多态性基因型与小儿结核病发病的风险关系。结果观察组rs422628位点3种基因型TT、TC和CC频率与对照组比较差异均无统计学意义(P>0.05),rs20541位点的AA基因型频率与对照组比较差异有统计学意义(P<0.05)。遗传模型分析中rs20541位点隐性模型的OR值为3.349(P=0.008),提示携带rs20541位点AA基因型的儿童患小儿结核病风险较高。同时携带rs422628位点CC基因型和rs20541位点AA基因型的儿童患小儿结核病风险较高(OR=28.500,P=0.043)。观察组血清IL-13水平高于对照组(P<0.05),且携带rs20541位点AA基因型患儿血清IL-13水平高于携带rs20541位点AG和GG基因型患儿(P<0.05)。结论携带IL-13基因rs20541位点AA基因型的儿童对小儿结核病的易感性较高,患病风险较大。Objective To investigate the relationship between GATA3,interleukin(IL)-13 gene polymorphism and IL-13 level and the susceptibility to tuberculosis in children.Methods A total of 414 children with tuberculosis who were treated in the hospital from December 2019 to December 2020 were randomly selected as the observation group.Another 456 cases of upper respiratory tract infection without pulmonary tuberculosis were selected as the control group.Venous blood DNA was extracted,and the rs422628 locus of GATA3 gene and rs20541 locus of IL-13 gene were detected by Taq-man fluorescence quantitative polymerase chain reaction technology.The level of IL-13 was detected by enzyme linked immunosorbent assay.The distribution frequency of different genotypes was calculated,and the relationship between different single nucleotide polymorphism genotypes and the incidence risk of tuberculosis in children was analyzed.Results There were no significant differences in TT,TC and CC genotypes of rs422628 locus between the observation group and the control group(P>0.05),and the difference in AA genotype of rs20541 locus between the observation group and the control group was statistically significant(P<0.05).In the genetic model analysis,the OR value of the recessive model of rs20541 locus was 3.349(P=0.008),which suggested that children carrying the AA genotype of rs20541 locus had a relatively high risk to tuberculosis.Children with both CC genotype of rs422628 locus and AA genotype of rs20541 locus were at relatively high risk to tuberculosis(OR=28.500,P=0.043).The serum level of IL-13 in the observation group was higher than that in the control group(P<0.05),and the serum level of IL-13 of children with AA genotype of rs20541 locus was higher than than of children with AG and GG genotypes of rs20541 locus(P<0.05).Conclusion Children with AA genotype of of rs20541 locus of IL-13 gene have relatively high susceptibility and high risk to tuberculosis.

关 键 词：GATA3 白细胞介素-13 小儿结核病 基因多态性 

分 类 号：R446.1[医药卫生—诊断学]