纳米银制备工艺优化及其生物活性  

Optimization for the preparation process of silver nanoparticles and their biological activity

作　　者：邹俊娜 罗文环 王珊[1] 王艳 ZOU Junna;LUO Wenhuan;WANG Shan;WANG Yan(Department of Pharmaceutical Engineering,College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China)

机构地区：[1]中南大学化学化工学院制药工程系,长沙410083

出　　处：《中南大学学报（医学版）》2022年第10期1398-1407,共10页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(2018JJ2491)。

摘　　要：目的:近年来采用生物法合成金属纳米粒子受到广泛关注,广大研究者试图寻找对环境污染小、易于制备的生物法来合成纳米银粒子(silver nanoparticles,AgNPs),并探究制备条件对合成AgNPs的影响。本研究旨在采用生物法合成粒径可控、效果良好的AgNPs,并对比其与化学法制备AgNPs的生物活性差异。方法:本研究分别以口蘑水提液(water extract of Tricholomagambosum,WET)、卷曲乳杆菌发酵上清液(cell-free supernatant of Lactobacillus crispatus,CFS)为还原剂及保护剂,AgNO_(3)溶液为前驱体,采用生物法制备AgNPs,同时采用柠檬酸钠化学法合成AgNPs。研究温度、pH、提取液用量、光照条件对生物法合成AgNPs的影响,并对所合成的AgNPs进行表征分析,最后对比上述3种AgNPs的抗菌效果以及对细胞的毒性和选择性。结果:各种表征证明上述3种方法都成功合成了AgNPs。2种生物法制备的AgNPs(WET-AgNPs和CFS-AgNPs)受pH及温度的影响较大,且都比化学法制备的AgNPs抑菌效果好(均P<0.01);其中,WET-AgNPs的抑菌效果比CFS-AgNPs略好。与化学法制备的AgNPs相比,WET-AgNPs和CFS-AgNPs对正常细胞的毒性低(均P<0.01),其中CFS-AgNPs在浓度为480μg/mL时细胞选择性较好。结论:WET和CFS可以合成具有生物活性的AgNPs,且与化学法制备的AgNPs有生物活性的差异。Objective:Recently,the use of biological synthesis of metal nanoparticles has attracted widespread attention.Researchers are trying to find a biological method to synthesize silver nanoparticles with little environmental pollution and easy preparation,and to explore the impact of preparation conditions on the synthesis of silver nanoparticles.This study aims to explore the biological synthesis of silver nanoparticles(AgNPs)with controllable size and good effect and to compare their biological activity with that of AgNPs prepared by chemical method.Methods:In this study,AgNPs were prepared by biological method with water extract of Tricholomagambosum(WET)and cell-free supernatant of Lactobacillus crispatus(CFS)as reducing agent and protective agent,and silver nitrate solution as precursor.Meanwhile,AgNPs was synthesized by sodium citrate chemical method.The effects of temperature,pH,dosage of extraction solution and light conditions on the biosynthesis of WET-AgNPs and CFS-AgNPs were investigated,and their characteristic of the synthesized WET-AgNPs and CFS-AgNPs were analyzed.Finally,the antibacterial effect,toxicity and selectivity of the 3 different AgNPs were compared.Results:AgNPs were synthesized successfully by the 3 methods with various characteristics.The AgNPs prepared by biological method(WET-AgNPs,CFS-AgNPs)were greatly affected by pH and temperature.The WET-AgNPs and CFS-AgNPs prepared by the biological methods had better antibacterial effect than the AgNPs by the chemical method(all P<0.01).Between them,the WET-AgNPs had a slightly higher antibacterial effect than the CFS-AgNPs.Compared with the AgNPs prepared by chemical method,the toxicity of the WET-AgNPs and CFS-AgNPs to normal cells was lower(both P<0.01),and the cell selectivity of the CFS-AgNPs was better when the concentration was 480μg/mL.Conclusion:AgNPs with biological activity can be synthesized from WET and CFS,which have different biological activity compared with the AgNPs prepared by biological method.

关 键 词：纳米银粒子 生物合成 表征 生物活性 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程] TQ131.22[医药卫生—基础医学]