Inhibition mechanisms of secretome proteins from Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus  

作　　者：Nurul Hana Zainal Baharin Nur Fadhilah Khairil Mokhtar Mohd Nasir Mohd Desa Nurul Diana Dzaraly AbdulRahman Muthanna Mazen M.Jamil Al-Obaidi Mohd Hafis Yuswan Sahar Abbasiliasi Norasfaliza Rahmad Wan Ahmad Kamil Wan Nur Ismah Amalia Mohd Hashim Shuhaimi Mustafa 

机构地区：[1]Laboratory of Halal Science Research,Halal Products Research Institute,Universiti Putra Malaysia,43400 UPM Serdang,Selangor,Malaysia [2]Department of Biomedical Sciences,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,43400 UPM Serdang,Selangor,Malaysia [3]Department of Science,University of Technology and Applied Sciences,Rustaq 10 P.C.329,Oman [4]Agro-Biotechnology Institute,Malaysia,National Institutes of Biotechnology Malaysia,43400 UPM Serdang,Selangor,Malaysia [5]Department of Microbiology,Faculty of Biotechnology and Biomolecular Science,Universiti Putra Malaysia,43400 UPM Serdang,Selangor,Malaysia

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第11期483-494,共12页亚太热带生物医学杂志（英文版）

基　　金：supported by the funds of Ministry of Higher Education,Malaysia and Universiti Putra Malaysia through Fundamental Research Grant Scheme (FRGS/1/2017/SKK11/UPM/01/1) and Putra Grant (GP/2017/9571800)

摘　　要：Objective:To determine the inhibition mechanisms of secretome protein extracted from Paenibacillus polymyxa Kp10(Kp10)and Lactococcus lactis Gh1(Gh1)against methicillin-resistant Staphylococcus aureus(MRSA)and vancomycin-resistant Enterococcus(VRE).Methods:The sensitivity and viability of MRSA and VRE treated with secretome proteins of Kp10 and Gh1 were determined using minimal inhibitory concentration,minimum bactericidal concentration,and time-to-kill assays.The morphological changes were observed using scanning electron microscopy and transmission electron microscopy.To elucidate the antimicrobial mechanism of secretome protein of Kp10 and Gh1 against MRSA and VRE,2D gel proteomic analysis using liquid chromatography-mass spectrometry was run by comparing upregulated and downregulated proteins,and the proton motive force study including the efflux of ATP,pH gradient,and the membrane potential study were conducted.Results:MRSA and VRE were sensitive to Kp10 and Gh1 secretome protein extracts and displayed apparent morphological and internal composition changes.Several proteins associated with cellular component functions were either downregulated or upregulated in treated MRSA and VRE by changing the membrane potential gradient.Conclusions:Kp10 and Gh1 secretome proteins reduce the growth of VRE and MRSA by damaging the cell membrane.Cell division,cell wall biosynthesis,and protein synthesis are involved in the inhibition mechanism.

关 键 词：ANTIMICROBIAL PROTEINS Secretome proteins ANTIBIOTIC-RESISTANCE Paenibacillus polymyxa Kp10 Lactococcus lactis Gh1 Vancomycin-resistant Enterococcus Methicillin-resistant Staphylococcus aureus Mechanism 

分 类 号：R378[医药卫生—病原生物学]