栓菌Trametes sp.LS-10C半固态发酵漆酶对吲哚的高效降解  

Efficient Degradation of Indole by Laccase from Trametes sp.Ls-10C Under Semi-solid Fermentation

作　　者：沈琪 秦梦茹 张呈彦 张慧云 孟娜[1,2,3] 李松 魏胜华[1,2,3] SHEN Qi;QIN Mengru;ZHANG Chengyan;ZHANG Huiyun;MENG Na;LI Song;WEI Shenghua(School of Biological and Food Engineering,Anhui Polytechnic University,Wuhu 241000,China;Anhui Engineering Technology Research Center of Microbial Fermentation,Wuhu 241000,China;Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding,Wuhu 241000,China)

机构地区：[1]安徽工程大学生物与食品工程学院,安徽芜湖241000 [2]微生物发酵安徽省工程研究中心,安徽芜湖241000 [3]安徽省工业微生物分子育种工程实验室,安徽芜湖241000

出　　处：《安徽工程大学学报》2022年第5期23-28,共6页Journal of Anhui Polytechnic University

基　　金：安徽省高等学校自然科学研究基金资助项目(KJ2021A0509);大学生创新创业训练计划基金资助项目(202010363052)。

摘　　要：为了有效地清除吲哚这一对人体具有严重危害的环境污染物,本研究以栓菌Trametes sp.LS-10C半固态发酵所产的漆酶为催化剂对吲哚进行降解反应。研究考察了介体的种类和浓度、酶加入量、降解体系的pH值、降解温度等因素对降解吲哚的影响。实验结果表明:在以浓度为3.2 mmol/L的丁香醛为介体、漆酶用量为0.12 U/mL、降解体系的pH值为4.5、降解温度为35℃的条件下,该菌所产漆酶对不同浓度吲哚(2.56 mmol/L、3.2 mmol/L和3.84 mmol/L)的降解率分别达到100%、90.2%和77.6%;对漆酶/丁香醛降解吲哚的动力学研究表明其最大反应速率V_(max)为0.22 mmol/(L·min),米氏常数K_(m)值为9.26 mmol/L。In order to effectively remove indole,an environmental pollutant with serious harm to human body,the degradation reaction of indole was carried out by using laccase produced by Trametes sp.LS-10C under semi-solid fermentation as catalyst.The factors affecting the degradation of indole were investigated.The experimental results showed that:under the conditions of mediator syringaldehyde concentration of 3.2 mmol/L,enzyme dosage of 0.12 U/mL,reaction pH 4.5 and reaction temperature 35℃,the degradation rates of different concentrations of indole(2.56 mmol/L,3.2 mmol/L and 3.84 mmol/L)by laccase were 100%,90.2%and 77.6%respectively.The kinetic study of the degradation of indole by laccase/syringal showed that the V_(max) was 0.22 mmol/(L·min),and the K_(m) value was 9.26 mmol/L.

关 键 词：栓菌 漆酶 吲哚 降解 

分 类 号：X703[环境科学与工程—环境工程]