鞍带石斑鱼精子诱导斜带石斑鱼雌核生殖  

INTRODUCTION OF DIPLOID GYNOGENESIS IN ORANGE-SPOT GROUPER EPINEPHELUS COIOIDES BY GIANT GROUPER EPINEPHELUS LANCEOLATUS SPERM

作　　者：樊欣 张维炜 吴廷昌[1] 文鑫 陈猛猛[1] 吴光灿 骆剑[1] FAN Xin;ZHANG Wei-Wei;WU Ting-Chang;WEN Xin;CHEN Meng-Meng;WU Guang-Can;LUO Jian(State Key Laboratory of Marine Resources Utilization in South China Sea,Hainan Aquatic Seed Industry Engineering Research Center,Hainan Academician Team Innovation Center,College of Ocean,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学海洋学院,海南省院士团队创新中心,海南省水产种业工程研究中心,南海海洋资源利用国家重点实验室,海口570228

出　　处：《水生生物学报》2022年第11期1675-1683,共9页Acta Hydrobiologica Sinica

基　　金：海南省重点研发计划项目(ZDYF2019045);海南省重大科技项目(ZDKJ2019011);海南省林浩然院士团队创新中心和淡水鱼类发育生物学国家重点实验室开放课题资助~~。

摘　　要：石斑鱼性成熟时间长和雌雄同体的特性延长了其育种的周期,采用人工雌核生殖策略能够显著提高选育效率。研究筛选了鞍带石斑鱼(Epinephelus lanceolatus)精子人工诱导斜带石斑鱼(Epinephelus coioides)雌核生殖的最佳条件,并通过形态学、流式细胞术和微卫星分子标记等方法进行鉴定。结果显示,灭活的异源精子可成功诱导斜带石斑鱼人工雌核生殖,精子灭活的最佳紫外照射强度为518 mJ/cm^(2);卵子受精后6min在4—6℃的条件下进行冷休克处理,可获得最优的40%的表观受精率(卵子正常分裂比率)和29.7%的孵化率,而未冷休克的对照组具有83.5%的表观受精率和80%的单倍体率。多重比较结果表明,在表观受精率方面人工诱导的时间差异(P=0.579)和水温差异(P=0.133)并不显著;而在孵化率上,处理时间组间差异极显著(P=0.002),而在水温组中差异不显著(P=0.396)。胚胎发育结果显示,人工雌核生殖诱导会导致孵化率降低,单倍体胚胎出现明显的发育障碍。微卫星分子标记鉴定结果显示,石斑鱼人工雌核生殖子代的遗传物质大部分来自于母本,同时也存在异精效应。The long period of sexual maturity and the characteristics of hermaphroditism extend the breeding cycle of grouper.In addition,the artificial gynogenesis strategy can significantly improve the breeding efficiency.In this study,we explored the best conditions for artificially inducing gynogenesis in Epinephelus coioides used by the sperm of Epinephelus lanceolatus,and were identified by morphology,flow cytometry and microsatellite markers.The results showed that artificial gynogenesis of Epinephelus coioides was successfully induced by inactivated heterologous sperm.The best suitable UV irradiation intensity of sperm was 518 mJ/cm2,and the irradiation time was 4min.6min after fertilization,cold shock treatmentcould obtain the optimal apparent fertilization rate of 40%and hatchability of 29.7%at 4—6℃,while the control group without cold shock showed 83.5%apparent fertilization rate and 80%haploid rate.The result of multiple comparison showed that in terms of apparent fertilization rate,there is no significant difference between the artificial induction time(P=0.579)and water temperature(P=0.133).When it comes to hatch rate,the difference of treatment time between groups is extremely significant(P=0.002),but not in water temperature group(P=0.396).The results of embryo development showed that the induction of artificial gynogenesis would lead to the decrease of partial hatchability and the haploid embryos behaved obvious developmental disorder.The results of microsatellite molecular markers indicated that most of the genetic material of the artificial gynogenetic progeny of grouper came from the female parent,and the heterospermia effect also exists.

关 键 词：雌核生殖 紫外照射 冷休克 微卫星标记 石斑鱼 

分 类 号：S961.2[农业科学—水产养殖]