三疣梭子蟹aqp2基因克隆及其在蜕皮中的功能  

CLONING OF AQP2 GENE OF PORTUNUS TRITUBERCULATUS AND ITS FUNCTION IN MOLTING

作　　者：吴捷 吕建建[2,3] 张伟伟 李玉坤 高保全 刘萍[2,3] WU Jie;LÜJian-Jian;ZHANG Wei-Wei;LI Yu-Kun;GAO Bao-Quan;LIU Ping(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fisheries Science,Qingdao 266071,China;Qingdao National Laboratory of Marine Science and Technology,Functional Laboratory of Marine Fishery Science and Food Production Process,Qingdao 266235,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,青岛266071 [3]青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,青岛266235

出　　处：《水生生物学报》2022年第11期1694-1700,共7页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划(2018YFD0901304);国家自然科学基金面上项目(41776160)资助。

摘　　要：为研究水通道蛋白AQP的生理功能,克隆了三疣梭子蟹(Portunus trituberculatus)水通道蛋白2基因(ptaqp2),该基因全长4126 bp,编码522个氨基酸,具有水通道蛋白基因家族的保守结构域及功能结构域;进化树聚类分析结果显示, ptaqp2基因属于水通道蛋白基因家族C-AQP类基因;组织表达结果显示, ptaqp2呈泛组织表达特征,在眼柄中的表达量最高,在肠和肌肉中也有较高表达,在血淋巴中的表达最低;ptaqp2基因在不同蜕皮时期呈显著差异表达(P<0.05),且在蜕皮前期表达量最高;在蜕皮激素(Molting Hormone简称MH)刺激下,该基因在肌肉、肠及胃中均呈不同程度的上调表达趋势;通过RNAi技术在蜕皮前期敲降ptaqp2的表达,发现能够显著推迟蜕皮过程。研究初步证明了ptaqp2基因在三疣梭子蟹蜕皮中发挥重要作用。In order to study the physiological function of aquaporin AQP,the aquaporin 2 gene(ptaqp2)of Portunus trituberculatus was cloned,which is full-length 4126 bp,encodes 522 amino acids and has the conserved domain and functional domain of aquaporin gene family.Cluster analysis showed that ptaqp2 gene belonged to C-AQP gene of aquaporin gene family.The results of tissue expression showed that the expression of ptaqp2 was the highest in eye stalk,the highest in intestine and muscle,and the lowest in hemolymph.The expression of ptaqp2 gene was significantly different in different molting stages,and the highest expression was in the early stage of molting.Under the stimulation of Molting Hormone(MH),the expression of ptaqp2 gene was up-regulated in muscle,intestine and stomach.By knocking down the expression of ptaqp2 in the early stage of molting by RNAi technique,it was found that it could significantly delay the molting process.It has been preliminarily proved that ptaqp2 gene plays an important role in molting of Portunus trituberculatus.

关 键 词：水通道蛋白 基因克隆 蜕皮激素 RNA干扰 三疣梭子蟹 

分 类 号：Q344.1[生物学—遗传学]