北海红树林内生真菌Aspergillus sp.GXIMD00016的抗炎次级代谢产物  

Anti-inflammatory secondary metabolites from a Beihai mangrove endophytic fungus Aspergillus sp.GXIMD00016

作　　者：肖泽恩 黄锡山 韦蔚烜 陆务玲 谭振 刘永宏 XIAO Ze′en;HUANG Xishan;WEI Weixuan;LU Wuling;TAN Zhen;LIU Yonghong(Institute of Marine Drugs/College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Zhuang Yao Medicine Center of Engineering and Technology,Nanning 530200,China;School of Chemistry and Pharmacy,Guangxi Normal University,Guilin 541000,China)

机构地区：[1]广西中医药大学海洋药物研究院/药学院,广西南宁530200 [2]广西壮瑶药工程技术研究中心,广西南宁530200 [3]广西师范大学化学与药学学院,广西桂林541000

出　　处：《中山大学学报（自然科学版）（中英文）》2022年第6期69-73,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广西科技基地和人才专项(桂科AD19245123);广西高校中青年教师科研基础能力提升项目(2021KY0295);广西中医药大学博士启动基金(2018BS030);大学生创新创业训练计划项目(S202110600111);广西中医药大学大学生科研训练课题(2021DXS11)。

摘　　要：采用大米培养基对红树内生真菌Aspergillus sp.GXIMD00016进行规模发酵,利用层析技术对发酵产物进行分离纯化,获得7个化合物。经波谱技术鉴定结构,并通过与文献对比鉴定化合物为aculeatusquinone A(1)、aculeatusquinone C(2)、roseopurpurin A(3)、myxotrichin C(4)、2,7-didechlorovicanic(5)、atraric acid(6)和2,5-dimethyl-1,3-benzenediol(7),其中化合物5为新天然产物,化合物3和4为首次从曲霉菌属中获得。首次考察化合物1~3和5~7对脂多糖(lipopolysaccharide,LPS)诱导RAW 264.7细胞产生NO的抑制作用,化合物1~3具有显著抗炎作用,IC_(50)值为16.68~28.15μmol/L。The mangrove endophytic fungus Aspergillus sp.GXIMD00016 was fermented by using rice medium.The metabolites were isolated by the chromatography technology and seven compounds were obtained.By analyzing MS and NMR spectroscopic data,their structures were identified as aculeatusquinone A(1),aculeatusquinone C(2),roseopurpurin A(3),myxotrichin C(4),2,7-didechlorovicanic(5),atraric acid(6)and 2,5-dimethyl-1,3-benzenediol(7).Besides,compound 5 was a new natural product,and compounds 3 and 4 were isolated from Aspergillus sp.for the first time.The inhibitory effects of compounds 1-3 and 5-7 on lipopolysaccharide(LPS)induced NO production were evaluated in mouse macrophage RAW264.7 cells.The results showed that compounds 1-3 had a significant anti-inflammatory activity with IC_(50) values ranging from 16.68 to 28.15μmol/L.

关 键 词：红树内生真菌 次级代谢产物 曲霉 抗炎 苯醌 

分 类 号：O629.9[理学—有机化学]