基于荧光探针的单细胞一氧化氮分析研究进展  

Research Progress of Single-cell Nitric Oxide Analysis Based on Fluorescent Probes

作　　者：姚惠文 郭小峰[1] 王红[1] YAO Huiwen;GUO Xiaofeng;WANG Hong(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072;College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065)

机构地区：[1]武汉大学化学与分子科学学院,湖北武汉430072 [2]湖北中医药大学药学院,湖北武汉430065

出　　处：《分析科学学报》2022年第5期635-640,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21777126,32070398,31670370);湖北省自然科学基金(2020CFB141)。

摘　　要：荧光分析法具有灵敏度高、选择性好、线性范围较宽、操作方便等优点,是生物、医学等研究领域强有力的分析工具。由于大多数生物活性物质本身没有荧光或荧光很弱,因此需要利用荧光探针对这些物质进行衍生,使之生成强荧光的物质,从而实现高灵敏检测。NO是生物体内重要的气体信号分子,广泛参与生物体的各种生理病理过程。单细胞NO分析有助于探究细胞的生物异质性,促进细胞免疫应答及相关疾病的发生发展过程的研究。本文从荧光探针与不同分析仪器(包括荧光显微镜、毛细管电泳和微流控芯片电泳)的结合入手,聚焦荧光探针的设计与开发,总结其在单细胞NO分析中的应用及最新进展。Fluorescence analysis provides potent tools for the biomedical research, with the advantages of high sensitivity, good selectivity, wide linear range, easy operation, etc.As most bioactive components do not fluoresce themselves or fluoresce very weakly, they should be converted to fluorescent derivatives with fluorescence probes to achieve highly sensitive detection.Nitric oxide(NO) is an important gaseous signaling molecule, which is widely involved in various physiological and pathological processes.Single-cell NO analysis contributes to the understanding of cellular heterogeneity and will promote the research of cellular immune response and the development of related diseases.This review highlights the recent progress of single-cell NO analysis based on fluorescence detection, mainly focus on the development of fluorescent probes and applications in combination with various analytical instruments(including fluorescence microscopy, capillary electrophoresis and microfluidic chip electrophoresis).

关 键 词：一氧化氮 单细胞 荧光探针 荧光成像 毛细管电泳 微流控芯片电泳 

分 类 号：O657.39[理学—分析化学]