电针不同腧穴对功能性腹泻大鼠多组织5-HT3R、5-HT4R的影响  

Effects of electroacupuncture at different acupoints on 5-HT3R and 5-HT4R in functional diarrhea model rats

作　　者：王晓燕[1] 张亚楠 韩晶[3] 李洋[1] 王萌萌 王琳 WANG Xiao-Yan;ZHANG Ya-Nan;HAN Jing(College of Acu-moxibustion and Massage of TCM of Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China)

机构地区：[1]山东中医药大学针灸推拿学院,山东济南250355 [2]山东中医药大学中医学院,山东济南250355 [3]山东中医药大学附属医院针灸科,山东济南250355 [4]山东中医药大学教务处,山东济南250355

出　　处：《中国老年学杂志》2022年第22期5566-5570,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金青年基金项目(No.81403326);山东省高等学校科技计划项目(No.J18KA253)。

摘　　要：目的观察功能性腹泻(FDr)模型大鼠的下丘脑、脊髓、胃、肠中5-羟色胺3受体(HT3R)、5-羟色胺4受体(HT4R)mRNA的表达,探讨电针治疗FDr的作用机制,并分析经穴脏腑相关性。方法将48只雄性SD大鼠随机分为正常组,模型组,足三里组,阴陵泉组,阴谷组、非经非穴组;每组8只。连续14 d用束缚、冷刺激、饮食失节等综合方法制备FDr模型。模型复制成功后,足三里组、阴陵泉组、阴谷组、非经非穴组给予电针干预20 min,1次/d,共7 d。测定大鼠胃内残留率与小肠推进率,并采取实时荧光定量PCR法检测下丘脑、脊髓、胃肠中5-HT3R、5-HT4R mRNA的表达。结果与正常组相比,造模后胃内残留率与小肠推进率均明显升高(P<0.05);模型组下丘脑、脊髓、胃肠中5-HT3R mRNA表达明显增加,5-HT4R mRNA表达明显减少(P<0.05)。电针后,足三里组和阴陵泉组与模型组相比,胃内残留率明显降低(P<0.05);下丘脑、脊髓、胃肠中5-HT3R mRNA表达明显减少,下丘脑、脊髓、胃窦中5-HT4R mRNA表达明显增加(P<0.05);与模型组相比,足三里组小肠推进率及结肠中5-HT4R mRNA表达明显升高(P<0.05)。与阴谷组、非经非穴组相比,足三里组胃内残留率、小肠推进率均明显降低(P<0.05);足三里组下丘脑、脊髓、胃、肠中5-HT3R mRNA表达明显减少,5-HT4R mRNA表达明显增加(P<0.05)。结论电针足三里穴和阴陵泉穴通过影响5-HT3R、5-HT4R mRNA表达对FDr起整体调节作用,提示经穴与脏腑之间存在特异性联系。Objective To observe the expression of 5-hydroxytryptamine 3 receptor(5-HT3R)and 5-hydroxytryptamine 4 receptor(5-HT4R)mRNA in hypothalamus,spinal cord,stomach and intestine of model rats with functional diarrhea(FDr),to explore the mechanism of electroacupuncture in treating FDr,and to analyze the correlation of acupoints.Methods 48 male SD rats were randomly divided into normal group,model group,Zusanli group,Yinlingquan group,Yingu group,non-meridian and non-acupoint group,with 8 rats in each group.FDr models were prepared by comprehensive method such as binding,cold stimulation and dietary disorders for 14 consecutive days.After successful replication of the model,Zusanli group,Yinling quan group and Yingu group,non-meridian and non-acupoint group were given electroacpuncture intervention,20 minutes each time,once a day,for 7 days.The gastric residual rate and intestinal propulsion rate were measured in rats,and the expression of 5-HT3R and 5-HT4R mRNA in hypothalamus,spinal cord,stomach and intestine were detected by real-time fluorescent quantitative PCR.Results Compared with normal group,the gastrivc residual rate and intestinal propulsion rate were significantly increased after modeling(P<0.05).In model group,the expression of 5-HT3R mRNA in hypothalamus,spinal cord,stomach and intestine were significantly increased,while the expression of 5-HT4R mRNA were significantly decreased(P<0.05).After electroacpuncture,the residual rate in Zusanli group and Yinlingquan group were significantly lower than those in model group(P<0.05).Compared with model group,the expression of 5-HT3R mRNA in hypothalamus,spinal cord,stomach and intestine were significantly decreased,while the expression of 5-HT4R mRNA in hypothalamus,spinal cord,stomach and intestine were significantly increased in Zusanli group and Yilingquan group(P<0.05).Compared with model group,the intestinal propulsion rate and 5-HT4R mRNA expression in the colon were significantly increased in Zusanli group(P<0.05).Compared with Yingu group,non-meridian and

关 键 词：功能性腹泻(FDr) 足三里穴 阴陵泉穴 5-羟色胺3受体(HT3R) 5-羟色胺4受体(HT4R) 

分 类 号：R245.97[医药卫生—针灸推拿学]