武汉地区汉族T2DM患者PPARγ2基因多态性与高脂血症易感性的相关性研究  

Study on the Correlation between PPARγ2 gene polymorphism and susceptibility to hyperlipidemia of Han nationality patients with T2DM in Wuhan area

作　　者：盛碧 夏晶 安靖 王雄 吴金虎 陈永刚 孟军华 陈灵 SHENG Bi;XIA Jing;AN Jing;WANG Xiong;WU Jinhu;CHEN Yonggang;MENG Junhua;CHEN Ling(Department of Pharmacy,the Third Hospital of Wuhan,Wuhan,Hubei 430060,China)

机构地区：[1]武汉市第三医院药学部,湖北武汉430060

出　　处：《国际检验医学杂志》2022年第22期2740-2744,共5页International Journal of Laboratory Medicine

基　　金：湖北省中央引导地方科技发展专项项目(2020ZXYD026);湖北省卫生健康委科研项目(WJ2021M011)。

摘　　要：目的 探讨武汉地区汉族2型糖尿病(T2DM)患者过氧化物酶体增殖物激活受体γ2(PPARγ2)基因多态性与该类人群发生高脂血症的关系。方法 选取2018年1月至2019年6月在武汉市第三医院行PPARγ2基因rs1801282位点基因多态性检测且无血缘关系的122例汉族T2DM患者为研究对象,收集患者的临床资料[年龄、性别、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、尿酸、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、糖化血红蛋白(HbA1c)]。根据是否并发高脂血症将患者分为两组,其中57例T2DM并发高脂血症患者作为研究组,65例T2DM患者作为对照组。采用荧光染色原位杂交技术检测PPARγ2基因rs1801282位点基因多态性。分析T2DM患者相关基因型分布特征,比较两组一般资料、PPARγ2基因rs1801282位点基因型及不同PPARγ2基因rs1801282位点基因型T2DM患者血脂水平。采用Logistic回归模型分析T2DM并发高脂血症的危险因素。结果 122例T2DM患者PPARγ2基因rs1801282位点基因型中,野生型CC 107例(87.7%),突变型CG 15例(12.3%),未检测出突变型GG,基因型分布符合Hardy-Weinberg遗传平衡定律(P>0.05)。研究组TC、TG、LDL、HbA1c水平高于对照组,HDL水平低于对照组,差异均有统计学意义(P<0.05)。不同PPARγ2基因rs1801282位点基因型T2DM患者血脂水平差异无统计学意义(P>0.05)。该地区汉族人群T2DM患者C、G等位基因分布频率为93.9%、6.1%,与欧洲人群的88.7%、11.3%比较,差异较大。PPARγ2基因rs1801282位点基因多态性不是T2DM患者并发高脂血症的独立危险因素(P>0.05)。结论 武汉地区汉族T2DM患者PPARγ2基因rs1801282位点基因型以CC型为主,其基因多态性与武汉地区汉族人群T2DM患者并发高脂血症无关。Objective To investigate the relationship between peroxisome proliferator-activated receptor γ2(PPARγ2) gene polymorphism and hyperlipidemia of Han nationality patients with type 2 diabetes mellitus(T2 DM) in Wuhan area.Methods A total of 122 unrelated Han T2 DM patients who underwent PPARγ2 gene rs1801282 gene polymorphism detection in Wuhan Third Hospital from January 2018 to June 2019 were selected as the research objects, and the clinical data [age, gender, total cholesterol(TC),triglyceride(TG),high density lipoprotein(HDL),low density lipoprotein(LDL),uric acid, alanine aminotransferase, aspartate aminotransferase, glycosylated hemoglobin(HbA1 c)] of the patients were collected.The patients were divided into two groups according to whether they were complicated with hyperlipidemia, 57 patients with T2 DM complicated with hyperlipidemia were selected as the study group, and 65 patients with T2 DM were selected as the control group.Fluorescence staining in situ hybridization technique with TL998 A fluorescence detection instrument was used to detect the rs1801282 gene polymorphism of PPARγ2 gene.The distribution characteristics of related genotypes of T2 DM patients were analyzed.The general data, PPARγ2 gene rs1801282 genotype and the blood lipid levels of T2 DM patients with different PPARγ2 gene rs1801282 genotypes were compared between the two groups.Logistic regression model was used to analyze the risk factors of T2 DM complicated with hyperlipidemia.Results Among the 122 T2 DM patients with PPARγ2 gene rs1801282 genotype, 107 patients(87.7%) were wild type CC,and 15 patients(12.3%) were mutant CG,and no mutant GG was detected.The distribution of genotypes was consistent with Hardy-Weinberg genetic equilibrium law(P>0.05).The levels of TC,TG,LDL and HbA1 c in the study group were higher than those in the control group, and the level of HDL in the study group was lower than that in the control group, and the differences were statistically significant(P<0.05).There were no statistically significant

关 键 词：过氧化物酶体增殖物激活受体γ2 基因多态性 2型糖尿病 高脂血症 

分 类 号：R587.1[医药卫生—内分泌] R589.2[医药卫生—内科学]