A型产气荚膜梭菌β2毒素蛋白间接ELISA抗体检测方法的建立  

Development of an Indirect ELISA Method for Detection of β2 Toxin Antibodies of Clostridium Perfringens Type A

作　　者：王林康 张玲 曾秀 戴益民[1] 吴允正 王婧祺 张锦华[1] WANG Linkang;ZHANG Ling;ZENG Xiu;DAI Yimin;WU Yunzheng;WANG Jingqi;ZHANG Jinhua(College of Animal Science and Technology,Jiangxi Agriculture University,Nanchang 330045,China)

机构地区：[1]江西农业大学动物科学技术学院,南昌330045

出　　处：《中国动物传染病学报》2022年第5期92-99,共8页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金资助项目(31860694);国家自然科学基金资助项目(31660720)。

摘　　要：为建立产气荚膜梭菌β2毒素抗体间接ELISA检测方法,本研究将产气荚膜梭菌β2毒素蛋白进行原核表达,用纯化的重组β2毒素蛋白作为包被抗原,建立了检测产气荚膜梭菌β2毒素抗体的间接ELISA方法。结果显示:该方法可以特异性地检测产气荚膜梭菌β2毒素抗体,与大肠杆菌、沙门菌和流行性腹泻病毒等常见猪腹泻病原的抗体均无交叉反应,并具有良好的灵敏度和重复性。本研究成功建立了特异性强、敏感性高以及重复性好的产气荚膜梭菌β2毒素抗体间接ELISA检测方法,为产气荚膜梭菌的流行病学调查以及疫苗接种后β2毒素抗体水平监测提供可靠的技术支持,同时也有助于产气荚膜梭菌β2毒素致病机制的研究。In order to develop an indirect ELISA method for detection ofβ2 toxin antibodies of Clostridium perfringens,the prokaryotic expression system was used to prepare recombinantβ2 toxin protein the coating antigen.The results showed that the indirect ELISA method specifically detected the antibodies againstβ2 toxin and had no cross-reaction with the antibodies against other common porcine diarrhea pathogens such as Escherichia coli,Salmonella and Porcine epidemic diarrhea virus.The indirect ELISA had good specificity,sensitivity and repeatability for detection of antibodies against Clostridium perfringensβ2 toxin,which provided reliable technical support for the epidemiological investigation and antibody monitoring post vaccination.

关 键 词：A型产气荚膜梭菌 β2毒素 间接ELISA 原核表达 

分 类 号：S852.61[农业科学—基础兽医学]