壳聚糖/丝素纳米纤维载药多层膜的构建和抗菌应用  

Construction and Antibacterial Application of Drug-loading Chitosan/Silk Nanofiber Multilayer Film

作　　者：刘思思 陈杰[3] 林祥德 柳华杰[3] 曾冬冬 Si-si Liu;Jie Chen;Xiang-de Lin;Hua-jie Liu;Dong-dong Zeng(Graduate School of Shanghai University of Traditional Chinese Medicine,Shanghai 201203;School of Medical Devices,Shanghai University of Medicine&Health Science,Shanghai 201318;School of Chemical Science and Engineering,Tongji University,Shanghai 200092)

机构地区：[1]上海中医药大学研究生院,上海201203 [2]上海健康医学院医疗器械学院,上海201318 [3]同济大学化学科学与工程学院,上海200092

出　　处：《高分子学报》2022年第12期1459-1465,共7页Acta Polymerica Sinica

基　　金：上海市自然科学基金(基金号19ZR1474300);国家自然科学基金(基金号22109117);中国博士后科学基金(基金号2021M692418);上海健康医学院科研导师制项目(项目号2020-4)资助。

摘　　要：构建抗菌表面涂层,是解决医疗器械表面因黏附生长细菌引发医源性感染的理想途径.因此,本研究利用层层自组装技术,以静电相互作用为驱动力制备了壳聚糖/丝素纳米纤维(CHI/SNF)多层膜,通过紫外-分光光度计(UV-Vis)追踪该多层膜的组装过程,并使用扫描电镜(SEM)对其进行形貌分析.然后,通过引入天然抗菌药物小檗碱(BBR)获得BBR-CHI/SNF多层膜.初步探讨了该载药多层膜的体外药物释放行为和对金黄色葡萄球菌和绿脓杆菌的抗菌性能.测试结果表明,多层膜的载药量能够通过调整膜的层数来控制.多层膜具备抗细菌黏附及一定抑菌性能,负载BBR后,通过BBR与多层膜内CHI的协同作用,抗菌性能得到了提升,抑制了金黄色葡萄球菌和绿脓杆菌的生长,其抑制率分别达到了(59.62±4.28)%和(51.65±3.77)%.总而言之,本研究构建了一种兼具抑菌和抗细菌黏附功能的载药多层膜,该多层膜制备简单,性能易于调控,基底灵活,因此在生物医用材料表面抗菌领域具有良好的应用前景.Construction of antibacterial surface coating is an ideal way to solve iatrogenic infection caused by adhesion growth bacteria on the surface of medical devices.Therefore,in this study,chitosan/silk nanofiber(CHI/SNF)multilayer film is prepared by layer-by-layer self-assembly technology with electrostatic interaction as the driving force.The assembly process of this multilayer film is traced by an ultraviolet spectrophotometer(UV Vis),and its morphology is analyzed using scanning electron microscopy(SEM).Then,BBR-CHI/SNF multilayer film is obtained by introducing the natural antibacterial berberine(BBR).The in vitro drug release behavior and antibacterial activity against S.aureus and P.aeruginosa.The test results show that the drug loading of the multilayer film can be controlled by adjusting the number of layers.The multilayer film has anti-bacterial adhesion and certain antibacterial performance.After loading BBR,through the synergistic effect of BBR and CHI in multilayer,the antibacterial performance is improved and the growth of S.aureus and P.aeruginosa is inhibited,with the inhibition rates of(59.62±4.28)%and(51.65±3.77)%,respectively.In conclusion,we have successfully constructed a drug loading multilayer film with both antibacterial and anti-bacterial adhesion functions.This multilayer film is easily prepared and its properties are easy to control and flexible substrate.Therefore,it has good prospects for application in the field of antibacterial on the surface of biomedical materials.

关 键 词：层层自组装 壳聚糖 丝素纳米纤维 小檗碱 抗菌 

分 类 号：TQ340.64[化学工程—化纤工业] TB383.1[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]