Bacterial profile and comparative antimicrobial efficacy of fresh urine of cows, buffaloes and humans  

作　　者：Bhoj R Singh Himani Agri Dhayananth Balusamy Varsha Jayakumar 

机构地区：[1]Division of Epidemiology,ICAR-Indian Veterinary Research Institute,Izatnagar-243122,India

出　　处：《Infectious Diseases Research》2022年第4期31-36,共6页传染病研究（英文）

摘　　要：Background:Holy cow(Bos indicus)urine is often considered a valuable therapeutic agent in India for treatment of many illnesses including infectious diseases.This study was conducted to assess the presence of potentially pathogenic bacteria and to determine antibacterial activity of cow urine vis a vis human and buffalo urine using standard bacteriological methods.Method:The study on 73 urine samples(Murra buffalo,Bubalus bubalis 13;Sahiwal cattle,Bos indicus 11;Tharparkar cattle,Bos indicus 18;Vrindavani cattle,a cross bred strain of Bos indicus and Bos taurus 12,and 19 humans),were collected aseptically and bacteriological analysis was done using standard clinical bacteriological methods.To determine antibacterial activity of filter-sterilized urine agar well diffusion and microplate broth dilution methods,against a battery of 18 strains,were used.Results:Revealed presence of at least 14 types of potentially harmful bacteria in fresh urine samples,Escherichia coli,was the most common detected in 13 samples followed by Hafnia alvei(11),Staphylococcus epidermidis(8),Bacillus mycoides(7),Proteus mirabilis(5),Enterococcus faecium(4),Acinetobacter calcoaceticus(3),Enterococcus faecalis(3),Paenibacillus pantothenticus(3),Salmonella enterica ssp.enterica Ser Enteritidis(3),Klebsiella pneumoniae ssp.pneumoniae(2),Pantoea agglomerans(2),Erwinia rhapontici(1),and Providencia rettgeri(1).The analysis for antimicrobial activity of filter-sterilized urine samples(73)against 18 test strains revealed that none of the urine could inhibit bacterial growth in agar well diffusion assay and 45 samples failed to inhibit any of the 18 bacteria with broth dilution assay.Those 28 urine samples which inhibited growth of one or more test bacteria were not bactericidal.For most of the test strains,there was no significant(P>0.05)difference in antibacterial potential of different urines irrespective of species,breed and sex of urine donors.However,S.epidermidis was inhibited by significantly(P<0.05)more human male urine samples than human

关 键 词：holy cow URINE antimicrobia Escherichia coli SALMONELLA ACINETOBACTER Klebsiella pneumoniae 

分 类 号：S82[农业科学—畜牧学]