银柴胡超临界CO_(2)萃取工艺优化及其萃取物成分分析  

Optimization of Supercritical CO_(2) Extraction Process and Component Analysis of Extract from Stellariae Radix

作　　者：宋乐 王红 李振凯 刘敦华[2] 彭励[1] SONG Le;WANG Hong;LI Zhenkai;LIU Dunhua;PENG Li(Ningxia University,School of Life Sciences,Yinchuan 750021,China;Ningxia University,School of Food and Wine,Yinchuan 750021,China)

机构地区：[1]宁夏大学生命科学学院,银川750021 [2]宁夏大学食品与葡萄酒学院,银川750021

出　　处：《中国现代应用药学》2022年第19期2498-2504,共7页Chinese Journal of Modern Applied Pharmacy

基　　金：宁夏重点研发计划项目(2021BEG02042);宁夏区自然基金项目(2021AAC03103)。

摘　　要：目的优化银柴胡脂质成分超临界CO_(2)萃取工艺并分析萃取物成分。方法采用Box-Behnken响应面法优化银柴胡脂质成分超临界CO_(2)萃取工艺,确定最佳工艺参数,并应用UHPLC-Q-Exactive Orbitrap MS对萃取物进行分析。结果经优化后最佳萃取条件:萃取压力35 MPa,萃取温度47℃,萃取时间3 h,在此条件下萃取得率为(0.332±1.31)%。鉴定分析结果显示银柴胡脂质成分含有甘油磷脂、鞘脂、甘油酯、固醇类、孕烯醇酮脂、脂肪酰、糖脂7大类,29亚类,2542个脂质分子,其中固醇类相对含量最高为88.29%,并首次发现银柴胡中含有植物甾醇酯、酵母甾醇及神经酰胺类成分。结论优化后的萃取工艺和成分分析结果可为银柴胡药效物质研究及其进一步开发利用奠定基础。OBJECTIVE To optimize the supercritical CO_(2)extraction process to extract lipid of Stellariae Radix and analyze its components.METHODS The supercritical CO_(2)extraction process of lipid of Stellariae Radix was optimized by Box-Behnken response surface methodology,and the optimal process parameters were determined.The lipid component was analyzed by UHPLC-Q-Exactive Orbitrap MS.RESULTS The best extraction process of lipid of Stellariae Radix was extraction pressure 35 MPa,extraction temperature 47℃,extraction time 3 h.Under this condition,the extraction yield of lipid was(0.332±1.31)%.Identification analysis results showed that the lipid component of Stellariae Radix contained 7 categories,including glycerophospho lipids,sphingolipids,glycerolipids,sterol lipids,pregnenolone lipids,fatty acyls,and glycolipids,29 subcategories,and 2542 lipid species.The relative content of sterollipids was up to 88.29%of the lipid.Furthermore,it was first discovered phytosterol esters,yeast sterols,and ceramides from Stellariae Radix.CONCLUSION The optimized extraction process and analysis of lipid components lay the foundation for the research and further development of Stellariae Radix medicinal substances.

关 键 词：银柴胡 超临界CO_(2)萃取 Box-Behnken响应面法 脂质 UHPLC-Q-Exactive Orbitrap MS 

分 类 号：R284.2[医药卫生—中药学]