畲药树参茎化学成分分析及抗炎活性部位高效液相指纹图谱的建立  

Analysis Chemical Constituents in the Stems of She Medicine Dendropanax Dentiger and Establishing HPLC Fingerprint of Its Anti-inflammatory Active Fraction

作　　者：刘敏 张晓芹[2] 黄绳武[3] 余乐 范蕾 陈梦 陈张金 陈桂云 陈琴鸣 顾正兵 LIU Min;ZHANG Xiaoqin;HUNG Shengwu;YU Le;FAN Lei;CHEN Meng;CHEN Zhangjin;CHEN Guiyun;CHEN Qinming;GU Zhengbing(Lishui Institute for Quality Inspection and Testing,Lishui 323000,China;Lishui Hospital of Traditional Chinese Medicine,Lishui 323000,China;Zhejiang Chinese Medical University,Hangzhou 310053,China;Taizhou Danding Biotechnology Co.,Ltd.,Taizhou 225400,China)

机构地区：[1]丽水市质量检验检测研究院,浙江丽水323000 [2]丽水市中医院,浙江丽水323000 [3]浙江中医药大学,杭州310053 [4]泰州丹鼎生物科技有限公司,江苏泰州225400

出　　处：《中国现代应用药学》2022年第19期2505-2510,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省药品监督管理局科技计划项目(2020003)。

摘　　要：目的对畲药树参茎所含化学成分进行确证分析,并建立抗炎活性部位的高效液相色谱指纹图谱。方法采用液相色谱-质谱联用、核磁共振波谱仪等分析仪器分离鉴定树参茎中的化学成分;采用高效液相色谱法建立抗炎活性部位指纹图谱:色谱柱为Waters Sunfire^(TM) C_(18)(4.6 mm×250 mm,5μm),流动相为乙腈-混合水溶液(含甲酸0.2%和四氢呋喃0.2%),梯度洗脱。检测波长为256 nm,柱温30℃,体积流量为1.0 mL·mim^(–1),分析时间为80 min,进样量为5μL。结果从树参茎中共分离鉴定出10个化合物,分别是绿原酸(1)、3,4-O-二咖啡酰基奎宁酸(2)、3,5-O-二咖啡酰基奎宁酸(3)、4,5-O-二咖啡酰基奎宁酸(4)、saikolignanoside A(5)、芦丁(6)、山柰酚-3-O-芸香糖苷(7)、紫丁香苷(8)、松柏醇(9)、芥子醛葡萄糖苷(10);12批样品抗炎活性部位指纹图谱相似度为0.901~0.992,均符合要求。结论树参茎中主要含有酚酸类、黄酮类、苯丙素类化合物;建立的指纹图谱不仅能体现树参茎抗炎的化学物质基础,而且鉴别方法简便、重复性好,可为树参茎的品质评价及主要抗炎活性成分的确证提供科学依据。OBJECTIVE To confirm and analyze the chemical constituents in the stems of Dendropanax dentiger,and establish the HPLC fingerprint of its anti-inflammatory active fraction.METHODS Compounds were isolated and identified by LC-MS,NMR and other analysis instruments.HPLC with Waters Sunfire^(TM) C_(18) column(4.6 mm×250 mm,5μm)was used.Mobile phase consisted acetonitrile-aqueous solution including 0.2%formic acid and 0.2%tetrahydrofuran with gradient elution.Detection wavelength was 256 nm.Column temperature was 30℃.Flow rate was 1.0 mL·mim^(–1)and analytic time was 80 min.Injection volume was 5μL.RESULTS Ten compounds were isolated and identified as chlorogenic acid(1),3,4-O-caffeoylquinic acid methyl ester(2),3,5-O-caffeoylquinic acid methyl ester(3),4,5-O-caffeoylquinic acid methyl ester(4),saikolignanoside A(5),rutin(6),kaempferol-3-O-rutinoside(7),syringin(8),coniferol(9),sinapaldehyde glucoside(10).Under the same fingerprint chromatogram condition,the methodological results were satisfactory to 12 batches of the anti-inflammatory active fractions.And the similarity range was 0.901−0.992.CONCLUSION The stems of Dendropanax dentiger mainly contain phenolic acids,flavonoids and phenylpropanoids.The established fingerprint can not only reflect the chemical basis of anti-inflammatory of the She medicine,but the identification method also is simple and reproducible.It can provide a scientific basis for the quality evaluation and the confirmation of main anti-inflammatory active components of the stems of Dendropanax dentiger.

关 键 词：树参茎 抗炎 活性部位 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]