重度子痫前期胎盘组织中YAP1、TAZ表达与围生结局的相关性  

Correlation between YAP1 and TAZ expression in placenta tissues of women with severe preeclampsia and their perinatal outcome

作　　者：焦娜[1] 贾源君 范丽丽[1] JIAO Na;JIA Yuanjun;FAN Lili(The First Affiliated Hospital of Henan University of Science and Technology,Luoyang,Henan Province,47100)

机构地区：[1]河南科技大学第一附属医院,洛阳471000

出　　处：《中国计划生育学杂志》2022年第11期2480-2484,共5页Chinese Journal of Family Planning

基　　金：2015年河南省医学科技攻关计划项目(201504026)。

摘　　要：目的:探讨重度子痫前期(PE)胎盘组织中YAP1、TAZ表达水平与围生结局的相关性。方法:收集2018年3月-2020年9月本院收治的220例PE孕妇为病例组,其中PE轻度组(102例)、PE重度组(118例);同期健康孕妇72例为对照组。采用RT-PCR法及免疫组化法测定胎盘YAP1、TAZ mRNA和蛋白水平。比较不同组孕妇YAP1、TAZ mRNA和蛋白表达水平差异。采用Pearson相关性分析探讨YAP1、TAZ mRNA和蛋白表达水平与PE严重程度、孕产妇妊娠结局的相关性。结果:对照组、PE轻度组、PE重度组YAP1、TAZ mRNA和蛋白表达依次降低(均P<0.05);对照组、PE轻度组、PE重度组YAP1、TAZ阳性率依次降低(均P<0.05)。PE严重程度与YAP1、TAZ mRNA和蛋白量均呈负相关(均P<0.05)。对照组、PE轻度组、PE重度组胎盘重量、新生儿出生体重和1min Apgar评分依次降低,且YAP1、TAZ mRNA和蛋白与胎盘重量、新生儿出生体重、1 min Apgar评分正相关(均P<0.05)。结论:重度PE胎盘组织中YAP1、TAZ表达降低,YAP1、TAZ低表达与围生不良结局具有相关性。Objective:To investigate the correlation between YAP1 and TAZ expression in placenta tissues of pregnant women with severe preeclampsia(PE)and their perinatal outcomes.Methods:220 pregnant women with PE were selected as the research objects from March 2018 to September 2020,which included 102 cases with mild preeclampsia in group A and 118 cases with severe preeclampsia in group B.And 72 healthy pregnant women were selected in group C during the same period.The mRNA and protein levels of YAP1 and TAZ in placenta tissue were measured by RT-PCR and immunohistochemistry.The mRNA and protein levels of YAP1 and TAZ of the women were compared among the three groups.Pearson correlation analysis was used to explore the correlation between the mRNA and protein expression levels of YAP1,TAZ of the women and the severity of their PE and their maternal pregnancy outcomes.Results:The mRNA and protein expressions of YAP1 and TAZ of the women in groups C,group A,and group B had decreased gradually(all P<0.05).The positive rates of YAP1 and TAZ of the women in group C,group A,and group B had decreased gradually(all P<0.05).The severity of PE of the women was negatively correlated with their mRNA and protein levels of YAP1 and TAZ(all P<0.05).The placental weight of the women,and the neonatal birth weight and 1 minute Apgar score in groups C,group A,and group B had decreased gradually,and the mRNA and protein levels of YAP1 and TAZ of the women were positively correlated with their placental weight and their neonatal birth weight and 1 minute Apgar score(all P<0.05).Conclusion:The expressions of YAP1 and TAZ in placenta tissue of the women with severe PE decrease,which are correlated with the adverse perinatal outcomes of these women.

关 键 词：重度子痫前期 胎盘 YAP1 TAZ:围生结局 

分 类 号：R714.244[医药卫生—妇产科学]