硅酸钙基生物活性陶瓷联用云南白药对炎症状态下牙髓干细胞矿化的影响  

Effects of iRoot BP Plus combined with Yunnan Baiyao on mineralization of dental pulp stem cells under inflammatory conditions

作　　者：赵莹 梁广智[2] 周昱其 王天琪 柳云霞[2] 刘晓影[3] 张娟娟[2] 孙岩[2] Zhao Ying;Liang Guangzhi;Zhou Yuqi;Wang Tianqi;Liu Yunxia;Liu Xiaoying;Zhang Juanjuan;Sun Yan(Affiliated Hospital of Weifang Medical University,Weifang 261035,Shandong Province,China;School of Stomatology,Weifang Medical University,Weifang 261000,Shandong Province,China;School of Life Science and Technology,Weifang Medical University,Weifang 261000,Shandong Province,China)

机构地区：[1]潍坊医学院附属医院,山东省潍坊市261035 [2]潍坊医学院口腔医学院,山东省潍坊市261000 [3]潍坊医学院生命科学与技术学院,山东省潍坊市261000

出　　处：《中国组织工程研究》2023年第19期2960-2967,共8页Chinese Journal of Tissue Engineering Research

摘　　要：背景:活髓切断术现作为永久保存活髓的治疗方法,术中选择合适的盖髓材料可在较大程度上增强其临床疗效并扩大其应用范围。目的:探讨云南白药、iRoot BP Plus及其联合应用对炎症状态下牙髓干细胞矿化的影响。方法:采用组织块法培养人原代牙髓干细胞,MTT法测定云南白药、iRoot BP Plus对牙髓干细胞增殖的影响,确定最佳干预质量浓度。将第3-6代牙髓干细胞分为5组:正常对照组、炎症对照组、云南白药组、iRoot BP Plus组、云南白药+iRoot BP Plus组,后4组使用1μg/mL脂多糖诱导2 h进行细胞造模,然后分别采用75μg/mL云南白药、100μg/mL iRoot BP Plus及两者联用进行刺激,采用碱性磷酸酶染色法、茜素红S染色法、Real time-PCR法、Western blot法检测药物对细胞矿化的影响。结果与结论:①与炎症对照组比较,云南白药、iRoot BP Plus及其联合应用处理后抑制了肿瘤坏死因子αmRNA的表达(P<0.001),云南白药+iRoot BP Plus组肿瘤坏死因子αmRNA的表达明显低于云南白药组、iRoot BP Plus组(P<0.001);②与炎症对照组比较,经云南白药、iRoot BP Plus及其联合应用处理后矿化结节数量、碱性磷酸酶活性显著增加,云南白药+iRoot BP Plus组矿化结节量、碱性磷酸酶活性高于云南白药组、iRoot BP Plus组(P<0.001);③与炎症对照组比较,经云南白药、iRoot BP Plus及其联合应用处理后DSPP、IBSP、MEPE mRNA的表达增加(P<0.001),云南白药+iRoot BP Plus组DSPP、IBSP、MEPE mRNA的表达高于云南白药组、iRoot BP Plus组(P<0.001);④与炎症对照组比较,云南白药组、云南白药+iRoot BP Plus组Akt、mTOR的磷酸化增加(P<0.001);⑤结果表明,云南白药、iRoot BP Plus均能促进牙髓干细胞矿化,两者联合应用矿化作用更显著;云南白药对牙髓干细胞的促矿化作用与Akt/mTOR通路相关。BACKGROUND:As a permanent preservation of living pulp,pulpotomy has been gradually applied in clinical treatment.The selection and application of pulpotomy materials can greatly enhance its clinical efficacy and expand its application range.OBJECTIVE:To study the effects of Yunnan Baiyao,iRoot BP Plus and their combination on the mineralization of pulp stem cells under the condition of inflammation.METHODS:Human primary dental pulp stem cells were cultured by tissue block method,and the effects of Yunnan Baiyao and iRoot BP Plus on the proliferation of dental pulp stem cells were determined by MTT assay to determine the optimal concentration of intervention quality.Passages 3-6 dental pulp stem cells were divided into five groups:normal control group,inflammatory control group,Yunnan Baiyao group,iRoot BP Plus group,and Yunnan Baiyao+iRoot BP Plus group.Cells in the inflammatory control group,Yunnan Baiyao group,iRoot BP Plus group,and Yunnan Baiyao+iRoot BP Plus group were treated with 1μg/mL lipopolysaccharide for 2 hours to establish models and then stimulated with 75μg/mL Yunnan Baiyao,100μg/mL iRoot BP Plus alone and their combination.Effects of drugs on cellular mineralization were determined by alkaline phosphatase assay,alizarin red S staining,real-time-PCR and western blot assay.RESULTS AND CONCLUSION:(1)Compared with the inflammatory control group,Yunnan Baiyao,iRoot BP Plus and their combination inhibited the mRNA expression of tumor necrosis factor-α(P<0.001).The mRNA expression of tumor necrosis factor-αin Yunnan Baiyao+iRoot BP Plus group was significantly lower than that in Yunnan Baiyao group and iRoot BP Plus group(P<0.001).(2)Compared with the inflammatory control group,the number of mineralized nodules and alkaline phosphatase activity were significantly increased after Yunnan Baiyao,iRoot BP Plus,and their combined application.The number of mineralized nodules and alkaline phosphatase activity in Yunnan Baiyao+iRoot BP Plus group were higher than those in Yunnan Baiyao and iRoot BP Plus g

关 键 词：牙髓炎 云南白药 iRoot BP Plus 活髓切断术 炎症 矿化 

分 类 号：R459.9[医药卫生—治疗学] R34[医药卫生—临床医学] R781.31