Dlx同源盒基因在牙源性细胞成骨向分化中的作用  

Roles of Distal-less homeobox genes in osteogenic differentiation of odontogenic cells

作　　者：曾曼曼 谭怀美 刘琰 Zeng Manman;Tan Huaimei;Liu Yan(Department of Stomatology,North Sichuan Medical College,Nanchong 637000,Sichuan Province,China;Department of Pediatrics,Women and Children’s Hospital of Chongqing Medical University,Chongqing 400000,China;Department of Prevention and Pediatric Dentistry,Hospital of Stomatology,Zunyi Medical University,Zunyi 563000,Guizhou Province,China)

机构地区：[1]川北医学院口腔医学系,四川省南充市637000 [2]重庆医科大学附属妇女儿童医院儿科,重庆市400000 [3]遵义医科大学附属口腔医院预防与儿童牙科,贵州省遵义市563000

出　　处：《中国组织工程研究》2023年第19期3083-3090,共8页Chinese Journal of Tissue Engineering Research

基　　金：2022年贵州省卫生健康委科学技术基金项目(gzwkj2022-167),项目负责人:刘琰。

摘　　要：背景:近年来研究发现Dlx同源盒基因在牙源性细胞成骨向分化诱导过程中发挥重要作用,可参与多种成骨相关信号通路的调控或影响成骨关键转录因子的表达,Dlx基因的这种调控作用可能为临床牙周组织再生研究提供新的思路和方法。目的:对Dlx基因家族主要成员在牙源性细胞成骨向分化中的作用及机制的研究进展进行综述。方法:以“distal-less homeobox gene,distal-less homeobox,dlx,dlx gene,bone-formation,osteogenesis,bone formation,ossification,ossifications,osteoclastogenesis,osteoclastogeneses,endochondral ossification,dental,oral,odontogenic,dental stem cell”及“Dlx同源盒基因,Dlx基因,Dlx,成骨,牙,牙源性,牙源性干细胞”为检索词,在PubMed、CNKI、万方数据库中进行相关文献检索,排除质量较低及与文章主题无关的研究及试验,最后纳入82篇文献进行仔细阅读分析与总结。结果与结论:①同源盒基因是决定机体生长发育的重要基因,同源盒基因由许多家族组成,只有一些基因家族在成骨中起作用,其中重要成骨基因家族是Dlx基因家族。牙胚的发育主要与2种同源盒基因有关:Msx基因家族和Dlx基因家族,后者在牙胚发育及牙源性细胞分化过程中起着重要的调控作用。②该研究肯定了Dlx3基因对BMP和Notch信号通路的正、负反馈调节作用,明确了Dlx3基因过表达对转录因子ZBTB16和EGR1参与的成骨诱导作用机制。③Dlx2/3/5均可在牙囊细胞中影响成骨,这可能是由于相似的同源域外部结构和染色体定位导致。④探究Dlx基因对牙组织作用时发现这些复杂的分子信号和细胞-细胞间相互有序作用化现象解释了神经嵴间充质或其结缔组织基质行为的扰动,从而导致许多口腔牙齿形态以及颌面部骨骼异常。因此,各类牙源性转录因子需要在时间和空间上以及在其定量输出方面受到严格的调节。⑤已有研究证实了Dlx基因家族在成骨�BACKGROUND:Recent studies have revealed that Distal-less(Dlx)homeobox genes play an important role in the induction of osteogenic differentiation of odontogenic cells and can participate in the regulation of various osteogenic-related signaling pathways or affect the expression of key osteogenic transcription factors.The regulatory effects of Dlx genes may provide new ideas and methods for clinical periodontal tissue regeneration studies.OBJECTIVE:To review research progress on the role and mechanisms of the Dlx gene family in the osteogenic differentiation of odontogenic cells.METHODS:Literature retrieval was conducted in PubMed,CNKI,and WanFang databases.The search terms were“distal-less homeobox gene,distal-less homeobox,Dlx,Dlx gene,bone-formation,osteogenesis,bone formation,ossification,ossifications,osteoclastogenesis,osteoclastogeneses,endochondral ossification,dental,oral,odontogenic,dental stem cell”in English and Chinese,respectively.Studies of low quality and irrelevant to the topic of the article were excluded.Finally,the included 82 articles were read,analyzed and summarized.RESULTS AND CONCLUSION:(1)Homeobox genes are important genes that determine the growth and development of the body.Homeobox genes are composed of many families.Among them,the important osteogenic gene family is the Dlx gene family.The development of tooth germ is mainly related to two homeobox genes:MSX gene family and Dlx gene family.The latter plays an important role in the development of tooth germ and the differentiation of odontogenic cells.(2)The positive and negative feedback regulation of Dlx3 gene on BMP and Notch signaling pathway,and the osteogenic induction mechanism of Dlx3 gene overexpression on transcription factors zbtb16 and EGR1 are clarified.(3)Dlx2/3/5 can affect osteogenesis in dental follicle cells,which may be due to similar homologous domain external structure and chromosome localization.(4)Exploring the effects of of Dlx genes on dental tissues revealed that these complex molecular signaling and cell-

关 键 词：Dlx同源盒基因 Dlx基因 DLX 成骨 牙 牙源性 牙源性干细胞 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R394.2