荧光共振能量转移聚合物胶束及其作为药物载体的研究进展  

Research progress on the fluorescence resonance energy transfer-based polymer micelles as drug carriers

作　　者：蒋林芮 曾妮 苗青山 吴昌强[2] 陕绍云(综述)[1] 苏红莹(审校)[1] JIANG Linrui;ZENG Ni;MIAO Qingshan;WU Changqiang;SHAN Shaoyun;SU Hongying(Department of Chemical Engineering,Kunming University of Science and Technology,Kunming 650500,P.R.China;Sichuan Key Laboratory of Medical Imaging,North Sichuan Medical College,Nanchong,Sichuan 637000,P.R.China)

机构地区：[1]昆明理工大学化学工程学院,昆明650500 [2]川北医学院医学影像四川省重点实验室,四川南充637000

出　　处：《生物医学工程学杂志》2022年第5期1022-1032,共11页Journal of Biomedical Engineering

基　　金：国家自然科学基金(51963013);云南省“万人计划”青年拔尖人才专项(YNWR-QNBJ-2019-085);医学影像四川省重点实验室(川北医学院)开放课题(SKLMI201902)。

摘　　要：双亲性聚合物自组装形成的胶束团聚体,因具有特殊的内核疏水、外壳亲水的核/壳结构及纳米尺寸,在药物传递、基因传递和生物传感器等领域具有广泛应用。然而,聚合物胶束的结构稳定性容易受到环境因素影响,如温度、pH、血液中存在的剪切力以及胶束表面修饰的基团与靶外细胞的相互作用等,作为药物载体材料应用时易发生药物泄露等问题。因此,胶束载体的结构完整性及体内分布行为研究对评价其治疗效果及临床应用可行性至关重要。目前,荧光共振能量转移(FRET)技术被广泛用于聚合物胶束的体内/外团聚、解离及分布情况的实时监测。本文对聚合物胶束、FRET技术的特点、FRET-聚合物胶束的结构及性质进行了简要介绍,着重综述了几类常用荧光探针对与聚合物胶束复合的机制,以及FRET-聚合物胶束作为药物载体的稳定性和体内/外分布行为研究进展,并对当前该技术面临的一些问题及可能的发展方向进行了概述。Polymer micelles formed by self-assembly of amphiphilic polymers are widely used in drug delivery,gene delivery and biosensors, due to their special hydrophobic core/hydrophilic shell structure and nanoscale. However,the structural stability of polymer micelles can be affected strongly by environmental factors, such as temperature, pH,shear force in the blood and interaction with non-target cells, leading to degradations and drug leakage as drug carriers.Therefore, researches on the structural integrity and in vivo distribution of micelle-based carriers are very important for evaluating their therapeutic effect and clinical feasibility. At present, fluorescence resonance energy transfer(FRET)technology has been widely used in real-time monitoring of aggregation, dissociation and distribution of polymer micelles(in vitro and in vivo). In this review, the polymer micelles, characteristics of FRET technology, structure and properties of the FRET-polymer micelles are briefly introduced. Then, methods and mechanism for combinations of several commonly used fluorescent probes into polymer micelles structures, and progresses on the stability and distribution studies of FRETpolymer micelles(in vitro and in vivo) as drug carriers are reviewed, and current challenges of FRET technology and future directions are discussed.

关 键 词：荧光共振能量转移技术 聚合物胶束 荧光探针对 药物载体 

分 类 号：TQ317[化学工程—高聚物工业] TQ460.4