织锦巴非蛤不同颜色斧足的转录组  

Transcriptome analysis of Paphia textile with different foot colors

作　　者：曾庆民[1] 赵虹博 邓素贞 章孝颖 刘贤德[2] ZENG Qingmin;ZHAO Hongbo;DENG Suzhen;ZHANG Xiaoying;LIU Xiande(Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fisheries Research Institute of Fujian,Xiamen 361013,China;Key Laboratory of Healthy Mariculture for the East China Sea,the Ministry of Agriculture and Rural Affairs,Fisheries College,Jimei University,Xiamen 361021,China)

机构地区：[1]福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建厦门361013 [2]集美大学水产学院,农业农村部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《水产学报》2022年第9期1562-1571,共10页Journal of Fisheries of China

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0901404)

摘　　要：为探索织锦巴非蛤斧足颜色差异的分子机制,以指导高品质织锦巴非蛤的选育工作,实验对织锦巴非蛤橘黄色和浅白色的斧足进行二代和三代转录组测序及差异表达分析。二代测序结果显示,6个样品共产生44.82 Gb clean data,GC含量为35.30%~38.21%,Q30碱基百分比均在94.43%以上。三代测序结果显示,橘黄色斧足组和浅白色斧足组分别得到16968和21611条高质量转录本,将其与各数据库进行比对,共获得5058条有注释信息的全长转录本。以三代全长转录本作为参考序列,将二代数据比对回参考序列,鉴定到57个差异表达转录本。其中,橘黄色斧足组相对于浅白色斧足组有34个差异转录本表达量上调,23个差异转录本表达量下调。使用实时荧光定量PCR(qRT-PCR)对随机挑选的12个差异表达转录本进行验证,其结果与转录组分析结果一致。随后,通过生物信息学分析发现,脂肪酸的生物合成和代谢、肌肉成分、能量代谢等都影响织锦巴非蛤斧足颜色的调控,其中过氧化物酶体增值物激活受体(PPARs)调节着脂肪酸的生物合成和代谢,肌钙蛋白是肌肉的重要组成成分,精氨酸激酶(AK)参与了色素富集过程中的能量供应。研究表明,PPARs、肌钙蛋白和AK在织锦巴非蛤色素富集过程中发挥着重要作用。This study aimed to explore the molecular mechanism of foot color difference to guide the selective breeding of high-quality Paphia textile.In this study,the foot transcriptomes of P.textile with different colors were analyzed by the second and third-generation sequencing technology.Analysis of gene differential expression was also performed in the orange and the white feet of P.textile.The second-generation sequencing results showed that a total of 44.82 Gb of clean data were obtained from six samples,GC content was 35.30%-38.21%,and the percentage of Q30 base was 94.43%or above.The results of third-generation sequencing showed that the orange foot group and light white group obtained 16968 and 21611 high quality transcripts,respectively.Among them,high quality transcript sequences were compared with the major databases,5058 full-length transcripts with annotated information were obtained.Three generation full-length transcripts were regarded as reference sequences,and the second-generation data were aligned back to the reference sequence.A total of 57 differentially expressed transcripts were obtained,including 34 up-regulated and 23 down-regulated genes in orange group.12 randomly selected transcripts were validated with realtime fluorescence quantitative PCR(qPCR),which showed that they were consistent with the qPCR analysis results.Subsequently,through bioinformatics analysis,it was found the regulation of foot color was affected by fatty acid biosynthesis and metabolism,muscle composition,energy metabolism energy metabolism in P.textile.Among them,peroxisome proliferators-activated receptors(PPARs)regulated fatty acid biosynthesis and metabolism.Troponin is an important component of muscle.Arginine kinase(AK)is involved in energy supply during pigment deposition.It is speculated that PPARs,Troponin,and AK play an important role in the pigment deposition.The results of this study may pave the way for further exploring the molecular mechanism of color-related genes and assist selective breeding practices in P

关 键 词：织锦巴非蛤 类胡萝卜素 斧足 转录组测序 差异表达转录本 

分 类 号：Q785[生物学—分子生物学] S968.35[农业科学—水产养殖]