一株松鼠葡萄球菌JS-1对鲫的致病性及其灭活疫苗保护作用  

Pathogenicity and inactivated vaccine protection of Staphylococcus sciuri JS-1 on Carassius auratus

作　　者：马爽 蒋弥 李艳红[1] 刘炀 孙瑜[1] 唐晓琪 吴正理[1] MA Shuang;JIANG Mi;LI Yanhong;LIU Yang;SUN Yu;TANG Xiaoqi;WU Zhengli(College of Fisheries,Research Center of Fishery Resources and Environment,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学水产学院,西南大学渔业资源环境研究中心,重庆400715

出　　处：《水产学报》2022年第9期1669-1679,共11页Journal of Fisheries of China

基　　金：国家自然科学基金(31770965);财政部和农业农村部-国家现代农业产业技术体系建设专项(CARS-49)。

摘　　要：为探究松鼠葡萄球菌JS-1对鲫的致病性及其灭活疫苗保护作用,本实验设计了注射感染实验、投喂感染实验和灭活疫苗保护实验,通过组织病理学观察、实时荧光定量PCR(qPCR)和酶活性检测等方法进行分析。结果显示,注射实验中鲫全身性出血、肝脏充血肿大,96 h LD_(50)为5×10^(6)CFU/mL。投喂含不同浓度JS-1的饲料后,鲫肝脏、脾脏、肾脏出现病变,细胞坏死;血清中转氨酶呈先增加后减少的趋势,高浓度(HG)组降低趋势最明显,溶菌酶活性显著升高;鲫肝脏和脾脏中TNF-α和IFN-γ表达量均先增加后减少,且显著高于对照组。注射灭活疫苗后,血清中溶菌酶活性显著高于对照组,而碱性磷酸酶、转氨酶、超氧化物歧化酶活性显著低于对照组,过氧化氢酶活性显著升高;经灭活疫苗免疫后的血清中,特异性IgM和总IgM与对照组相比都显著增加,注射感染JS-110 d后鲫的存活率达83.3%。研究表明,松鼠葡萄球菌JS-1对鲫的致病性强,其灭活疫苗可为鲫提供有效的保护,为松鼠葡萄球菌疫苗的开发提供了理论基础。Staphylococcus sciuri is a common zoonotic pathogen to humans,animals and fish.It can cause a variety of diseases and a high fatality rate to aquatic animals.Carassius auratus is one of the most important aquaculture species in the world,and can be used as a good aquatic animal model.In order to explore the pathogenicity and inactivated vaccine protectivity of S.sciuri JS-1 on C.auratus,we designed the JS-1 injected infection experiment,the JS-1 supplemented feeding experiment,and the inactivated vaccine protection experiment,and samples were detected by histopathological observation,quantitative real-time PCR(qPCR)and enzyme activity detection.Results showed that in the JS-1 injected infection samples,C.auratus showed systemic hemorrhage,and its liver was congested and enlarged,and the 96-hour LD_(50) was 5×10^(6) CFU/mL.In the JS-1 supplemented feeding experiment,the liver of C.auratus showed congestion and enlargement with irregular nucleus shape,the spleen was congested and had serious necrosis,the renal corpuscle was swollen and the space of renal capsule was enlarged,and the transaminase in serum increased at first and then decreased,and reached the highest level on day 14.The high concentration group showed the most obvious decrease trend,and the activity of lysozyme was significantly increased,compared with control group.On day 14,the lysozyme activity of the high concentration group was significantly higher than that of the control group(P<0.05),on day 28,the lysozyme activity of the low concentration group was significantly increased(P<0.05).The expression of TNF-αand IFN-γgenes in the liver and spleen of C.auratus increased at first and then decreased,and was significantly higher than that of the control group,and on day 21,the difference still existed.After injection of the inactivated vaccine,the activity of lysozyme in serum was significantly higher than that in the control group,while activities of alkaline phosphatase,transaminase,and superoxide dismutase were significantly lower than those in

关 键 词：鲫 松鼠葡萄球菌 致病性 免疫基因表达 非特异性免疫 灭活疫苗 

分 类 号：S941.42[农业科学—水产养殖]